The DNA-repair Ku70 protein is located in the nucleus and tail of elongating spermatids in grasshoppers.

Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology Pub Date : 2007-01-01 Epub Date: 2007-11-06 DOI:10.1007/s10577-007-1183-5

Josefa Cabrero, Rogelio J Palomino-Morales, Juan Pedro M Camacho

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引用次数: 17

Abstract

Fluorescence immunostaining for the phosphorylated H2AX histone (gammaH2AX) in the grasshopper Eyprepocnemis plorans has shown abundance of gammaH2AX in the nuclei of round and elongating spermatids, suggesting that DNA double-strand breaks (DSBs) occur regularly during spermiogenesis. Immunofluorescence patterns for Ku70, a DNA-repair protein participating in the non-homologous end-joining (NHEJ) pathway, showed that this protein is present in round and elongating spermatids, implying that the NHEJ DNA-repair pathway operates during chromatin compaction in spermiogenesis. In addition, during the final stages of spermiogenesis, the Ku70 protein concentrates on the region forming the sperm tail. Since Ku70 was also abundant in spermatid tails, it is reasonable to assume that Ku70 might play a novel function in sperm-tail formation. The analysis of Ku70 immunofluorescence patterns in 13 other grasshopper species also showed the presence of this protein in the nucleus and tail of elongating spermatids, indicating that this is a general characteristic in grasshoppers.

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dna修复蛋白Ku70位于蝗虫伸长精细胞的细胞核和尾部。

荧光免疫染色显示，蚱蜢的圆形和细长精子细胞核中有大量磷酸化的H2AX组蛋白(gammaH2AX)，这表明DNA双链断裂(DSBs)在精子发生过程中有规律地发生。参与非同源末端连接(NHEJ)途径的dna修复蛋白Ku70的免疫荧光模式显示，该蛋白存在于圆形和细长的精子中，这意味着NHEJ dna修复途径在精子发生过程中染色质压缩过程中起作用。此外，在精子发生的最后阶段，Ku70蛋白集中在形成精子尾部的区域。由于Ku70在精尾中也大量存在，我们有理由认为Ku70可能在精尾形成过程中发挥了新的功能。对其他13种蚱蜢的Ku70免疫荧光图谱分析也表明，该蛋白存在于伸长精子的细胞核和尾部，表明这是蚱蜢的普遍特征。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology

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