Centrifugal seeding increases seeding efficiency and cellular distribution of bone marrow stromal cells in porous biodegradable scaffolds.

Jason D Roh, Gregory N Nelson, Brooks V Udelsman, Matthew P Brennan, Britt Lockhart, Peter M Fong, Reynold I Lopez-Soler, W Mark Saltzman, Christopher K Breuer
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引用次数: 91

Abstract

Bone marrow stromal cells (MSCs) are a promising cell source for a variety of tissue engineering applications, given their ready availability and ability to differentiate into multiple cell lineages. MSCs have been successfully used to create neotissue for cardiovascular, urological, and orthopedic reconstructive surgical procedures in preclinical studies. The ability to optimize seeding techniques of MSCs onto tissue engineering scaffolds and the ability to control neotissue formation in vitro will be important for the rational design of future tissue engineering applications using MSCs. In this study we investigated the effect of centrifugal force on seeding MSCs into a biodegradable polyester scaffold. MSCs were isolated and seeded onto porous scaffold sections composed of nonwoven polyglycolic acid mesh coated with poly(L-lactide-co-epsilon-caprolactone). Compared to standard static seeding techniques, centrifugal seeding increased the seeding efficiency by 38% (p < 0.007) and significantly improved cellular distribution throughout the scaffold. Overall, centrifugal seeding of MSCs enhances seeding efficiency and improves cellular penetration into scaffolds, making it a potentially useful technique for manipulating neotissue formation by MSCs for tissue engineering applications.

离心播种提高了骨髓基质细胞在多孔可生物降解支架中的播种效率和细胞分布。
骨髓基质细胞(MSCs)由于其现成的可用性和分化成多种细胞系的能力,在各种组织工程应用中是一种很有前途的细胞来源。在临床前研究中,间充质干细胞已成功用于心血管、泌尿科和骨科重建外科手术中创建新组织。优化MSCs在组织工程支架上的播种技术以及在体外控制新组织形成的能力对于合理设计未来使用MSCs的组织工程应用具有重要意义。在这项研究中,我们研究了离心力对MSCs植入生物可降解聚酯支架的影响。分离MSCs并将其植入多孔支架切片上,该支架切片由涂有聚l -乳酸-共epsilon-己内酯的无纺布聚乙醇酸网组成。与标准的静态播种技术相比,离心播种提高了38%的播种效率(p < 0.007),并显著改善了细胞在整个支架中的分布。总的来说,MSCs的离心播种提高了播种效率,并改善了细胞对支架的渗透,使其成为一种潜在的有用技术,用于组织工程应用中操纵MSCs形成新组织。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Tissue engineering
Tissue engineering CELL & TISSUE ENGINEERING-BIOTECHNOLOGY & APPLIED MICROBIOLOGY
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