IN VITRO EFFECTS OF GUINEA PIG SERUM ON THE JENSEN, JA-1 AND JA-2 SARCOMAS.

Abstract

Summary The in vitro growth response of the Jensen sarcoma and its nutritional variants (JA-1 and JA-2) to media containing normal guinea pig serum and partially purified asparaginase and media devoid of asparagine was studied. A comparison of the morphology of the cells grown in the presence of guinea pig serum and media devoid of asparagine showed a remarkable similarity. Further, the growth characteristics of cultures exposed to guinea pig serum and partially purified asparaginase were essentially the same. These results suggested the active component of guinea pig serum was asparaginase which catalyzed the destruction of extracellular asparagine.