Effect of Extractum cepae, heparin sodium and allantoin combination (ContractubexTM) on adhesion and fibrosis after neck surgery (thyroidectomy) in rats.
Sener Balas, Serhat Tokgoz, Kerim Bora Yilmaz, Evrim Onder, Harun Karabacak, Melih Akinci, Bahattin Bayar, Attila Isgoren
{"title":"Effect of Extractum cepae, heparin sodium and allantoin combination (Contractubex<sup>TM</sup>) on adhesion and fibrosis after neck surgery (thyroidectomy) in rats.","authors":"Sener Balas, Serhat Tokgoz, Kerim Bora Yilmaz, Evrim Onder, Harun Karabacak, Melih Akinci, Bahattin Bayar, Attila Isgoren","doi":"10.14744/nci.2021.80000","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>Reoperative thyroid surgery is technically difficult process with increased complications due to the adhesions and fibrosis caused by the previous surgery. In this experimental animal model, we planned to investigate the effect of Contracttubex<sup>TM</sup>, a mixture of Extractum cepae, Heparin sodium and Allantoin, on adhesion and fibrosis after neck surgery (thyroidectomy).</p><p><strong>Methods: </strong>The current study is an experimental animal model of post-thyroidectomy adhesion. Twelve Wistar-Albino male rats in two groups were used. Under sterile conditions, a midline incision on the neck was made. The anterior thyroid muscles were separated and the thyroid lodge was reached. As a minor interventional procedure, a sponge was applied to the thyroid tissue, and then a combination of 1 g Extractum capae, heparin, sodium, and allantoin was applied to the dissection site in the drug group. Rats in both groups were sacrificed on 30<sup>th</sup> day. Cervical regions were dissected and evaluated for macroscopic adhesion. Tissue samples were taken for microscopic evaluation for fibrosis and inflammation.</p><p><strong>Results: </strong>In the experimental group in which Contractubex<sup>TM</sup> was applied, inflammation was not detected in five (83.3%) of six rats while no inflammation was detected in four (66.7%) of six rats in the control group (p=0.505). Considering the fibrosis score, mild or moderate fibrosis was detected in four (66.7%) of six rats in the control group, while fibrosis was found in only two (33.3%) rats in the experimental group (p=0.264). When macroscopic adhesion was evaluated, two (33.3%) rats of the control group subjects were Grade 4, and one (16.7%) was Grade 3. No rats in the experimental group were Grade 4 (p=0.392).</p><p><strong>Conclusion: </strong>Contractubex<sup>TM</sup> seems to be effective in preventing adhesions and fibrosis after thyroidectomy and neck surgery, but further research is needed for use in human studies.</p>","PeriodicalId":19164,"journal":{"name":"Northern Clinics of Istanbul","volume":"9 5","pages":"495-500"},"PeriodicalIF":0.9000,"publicationDate":"2022-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/32/79/NCI-9-495.PMC9677052.pdf","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Northern Clinics of Istanbul","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.14744/nci.2021.80000","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2022/1/1 0:00:00","PubModel":"eCollection","JCR":"Q3","JCRName":"MEDICINE, GENERAL & INTERNAL","Score":null,"Total":0}
引用次数: 0
Abstract
Objective: Reoperative thyroid surgery is technically difficult process with increased complications due to the adhesions and fibrosis caused by the previous surgery. In this experimental animal model, we planned to investigate the effect of ContracttubexTM, a mixture of Extractum cepae, Heparin sodium and Allantoin, on adhesion and fibrosis after neck surgery (thyroidectomy).
Methods: The current study is an experimental animal model of post-thyroidectomy adhesion. Twelve Wistar-Albino male rats in two groups were used. Under sterile conditions, a midline incision on the neck was made. The anterior thyroid muscles were separated and the thyroid lodge was reached. As a minor interventional procedure, a sponge was applied to the thyroid tissue, and then a combination of 1 g Extractum capae, heparin, sodium, and allantoin was applied to the dissection site in the drug group. Rats in both groups were sacrificed on 30th day. Cervical regions were dissected and evaluated for macroscopic adhesion. Tissue samples were taken for microscopic evaluation for fibrosis and inflammation.
Results: In the experimental group in which ContractubexTM was applied, inflammation was not detected in five (83.3%) of six rats while no inflammation was detected in four (66.7%) of six rats in the control group (p=0.505). Considering the fibrosis score, mild or moderate fibrosis was detected in four (66.7%) of six rats in the control group, while fibrosis was found in only two (33.3%) rats in the experimental group (p=0.264). When macroscopic adhesion was evaluated, two (33.3%) rats of the control group subjects were Grade 4, and one (16.7%) was Grade 3. No rats in the experimental group were Grade 4 (p=0.392).
Conclusion: ContractubexTM seems to be effective in preventing adhesions and fibrosis after thyroidectomy and neck surgery, but further research is needed for use in human studies.
目的:甲状腺再手术是一个技术难度大的手术过程，由于前一次手术引起的粘连和纤维化，并发症增加。在这个实验动物模型中，我们计划研究cep提取物、肝素钠和尿囊素的混合物ContracttubexTM对颈部手术(甲状腺切除术)后粘连和纤维化的影响。方法:建立甲状腺切除术后粘连的实验动物模型。Wistar-Albino雄性大鼠12只，分为两组。在无菌条件下，在颈部中线切开。分离甲状腺前肌，到达甲状腺窝。作为一个小的介入程序，在甲状腺组织上敷海绵，然后在药物组的解剖部位联合应用1 g cap提取物、肝素、钠和尿囊素。两组大鼠均于第30天处死。解剖颈椎区域并评估宏观粘连。取组织样本进行纤维化和炎症的显微评估。结果:实验组6只大鼠中5只(83.3%)无炎症反应，对照组6只大鼠中4只(66.7%)无炎症反应(p=0.505)。从纤维化评分来看，对照组6只大鼠中有4只(66.7%)出现轻度或中度纤维化，而实验组只有2只(33.3%)出现纤维化(p=0.264)。肉眼粘连评价时，对照组2只(33.3%)为4级，1只(16.7%)为3级。实验组无4级大鼠(p=0.392)。结论:ContractubexTM对甲状腺切除术和颈部手术后的粘连和纤维化有一定的预防作用，但在人体内的应用还需要进一步的研究。
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
