{"title":"The ethanol extract of Periplaneta Americana L. improves ulcerative colitis induced by a combination of chronic stress and TNBS in rats.","authors":"Jing-Na Zhang, Min-Zhe Sun, Heng Liu, Han-Chao Zhang, Huai Xiao, Yu Zhao, Chenggui Zhang, Hai-Rong Zhao","doi":"10.1590/acb370505","DOIUrl":null,"url":null,"abstract":"<p><strong>Purpose: </strong>To investigate the effects of Periplaneta americana L. on ulcerative colitis (UC) induced by a combination of chronic stress (CS) and 2,4,6-trinitrobenzene sulfonic acid enema (TNBS) in rats.</p><p><strong>Methods: </strong>The experiment UC model with CS was established in rats by a combination of chronic restraint stress, excess failure, improper, and TNBS. The body weight, disease activity index (DAI), colonic mucosal injury index (CMDI), histopathological score (HS) and pro-inflammatory mediators were measured. The content of corticotropin-releasing hormone (CRH) in hypothalamus or adrenocorticotropic hormone (ACTH) and corticosteroids (CORT) in plasma were evaluated by enzyme-linked immunosorbent assay. The proportion of T lymphocyte subsets was detected by flow cytometry, and gut microbiota was detected by 16S rDNA amplicon sequencing.</p><p><strong>Results: </strong>Weight loss, DAI, CMDI, HS and proinflammatory mediators were reversed in rats by P. americana L. treatment after UC with CS. Increased epidermal growth factor (EGF) was observed in P. americana L. groups. In addition, P. americana L. could reduce the content of CRH and ACTH and regulate the ratio of CD3+, CD3+CD8+ and CD3+CD4+CD25+/CD4+ in spleen. Comparably, P. americana L. changes composition of gut microbiota.</p><p><strong>Conclusions: </strong>The ethanol extract of Periplaneta Americana L. improves UC induced by a combination of CS and TNBS in rats.</p>","PeriodicalId":6992,"journal":{"name":"Acta cirurgica brasileira","volume":"37 5","pages":"e370505"},"PeriodicalIF":1.1000,"publicationDate":"2022-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9377204/pdf/","citationCount":"4","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Acta cirurgica brasileira","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1590/acb370505","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2022/1/1 0:00:00","PubModel":"eCollection","JCR":"Q3","JCRName":"SURGERY","Score":null,"Total":0}
引用次数: 4
Abstract
Purpose: To investigate the effects of Periplaneta americana L. on ulcerative colitis (UC) induced by a combination of chronic stress (CS) and 2,4,6-trinitrobenzene sulfonic acid enema (TNBS) in rats.
Methods: The experiment UC model with CS was established in rats by a combination of chronic restraint stress, excess failure, improper, and TNBS. The body weight, disease activity index (DAI), colonic mucosal injury index (CMDI), histopathological score (HS) and pro-inflammatory mediators were measured. The content of corticotropin-releasing hormone (CRH) in hypothalamus or adrenocorticotropic hormone (ACTH) and corticosteroids (CORT) in plasma were evaluated by enzyme-linked immunosorbent assay. The proportion of T lymphocyte subsets was detected by flow cytometry, and gut microbiota was detected by 16S rDNA amplicon sequencing.
Results: Weight loss, DAI, CMDI, HS and proinflammatory mediators were reversed in rats by P. americana L. treatment after UC with CS. Increased epidermal growth factor (EGF) was observed in P. americana L. groups. In addition, P. americana L. could reduce the content of CRH and ACTH and regulate the ratio of CD3+, CD3+CD8+ and CD3+CD4+CD25+/CD4+ in spleen. Comparably, P. americana L. changes composition of gut microbiota.
Conclusions: The ethanol extract of Periplaneta Americana L. improves UC induced by a combination of CS and TNBS in rats.
目的:探讨美洲大蠊对慢性应激(CS)联合2,4,6-三硝基苯磺酸灌肠(TNBS)所致大鼠溃疡性结肠炎(UC)的影响。方法:采用慢性约束应激、过度衰竭、不当和TNBS联合作用建立大鼠实验性UC模型。测定大鼠体重、疾病活动指数(DAI)、结肠黏膜损伤指数(CMDI)、组织病理学评分(HS)和促炎介质。采用酶联免疫吸附法测定下丘脑促肾上腺皮质激素释放激素(CRH)或血浆促肾上腺皮质激素(ACTH)和皮质类固醇(CORT)含量。流式细胞术检测T淋巴细胞亚群比例，16S rDNA扩增子测序检测肠道菌群。结果:大鼠UC合并CS后，美洲紫杉树对大鼠的体重减轻、DAI、CMDI、HS及促炎介质均有逆转作用。美洲紫菀组表皮生长因子(EGF)升高。此外，美洲蓟还能降低脾脏中CRH和ACTH的含量，调节脾脏中CD3+、CD3+CD8+和CD3+CD4+CD25+/CD4+比值。相比之下，美洲p.a americana L.改变肠道微生物群的组成。结论:美洲大蠊乙醇提取物对CS和TNBS联合诱导的大鼠UC有改善作用。
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