{"title":"Towards the development of phytoextract based healthy ageing cognitive booster formulation, explored through <i>Caenorhabditis elegans</i> model.","authors":"Rakesh Pandey, Dayanandan Mani, Karuna Shanker, Dnyaneshwar Umrao Bawankule, Debabrata Chanda, Raj Kishori Lal, Anirban Pal, Puja Khare, Narendra Kumar, Sudeep Tandon, Dharmendra Saikia, Anil Kumar Gupta, Ramesh Kumar Srivastava, Sanjay Kumar, Ram Suresh, Saudan Singh, Alok Kalra, Anil Maurya, Dewasya Pratap Singh, Taruna Pandey, Shalini Trivedi, Shachi Suchi Smita, Akanksha Pant, Laxmi Rathor, Jyotsna Asthana, Mashu Trivedi, Prabodh Kumar Trivedi","doi":"10.1007/s13237-022-00407-1","DOIUrl":null,"url":null,"abstract":"<p><p>The positive effect of herbal supplements on aging and age-related disorders has led to the evolution of natural curatives for remedial neurodegenerative diseases in humans. The advancement in aging is exceedingly linked to oxidative stress. Enhanced oxidative stress interrupts health of humans in various ways, necessitating to find stress alleviating herbal resources. Currently, minimal scientifically validated health and cognitive booster resources are available. Therefore, we explored the impact of plant extracts in different combinations on oxidative stress, life span and cognition using the multicellular transgenic humanized <i>C. elegans</i>, and further validated the same in <i>Mus musculus</i>, besides testing their safety and toxicity. In our investigations, the final product-the HACBF (healthy ageing cognitive booster formulation) thus developed was found to reduce major aging biomarkers like lipofuscin, protein carbonyl, lipid levels and enhanced activity of antioxidant enzymes. Further confirmation was done using transgenic worms and RT-PCR. The cognitive boosting activities analyzed in <i>C. elegans</i> and <i>M. musculus</i> model system were found to be at par with donepezil and L-dopa, the two drugs which are commonly used to treat Parkinson's and Alzheimer's diseases. In the transgenic <i>C. elegans</i> model system, the HACBF exhibited reduced aggregation of misfolded disease proteins α-synuclein and increased the health of nicotinic acetylcholine receptor, levels of Acetylcholine and Dopamine contents respectively, the major neurotransmitters responsible for memory, language, learning behavior and movement. Molecular studies clearly indicate that HACBF upregulated major genes responsible for healthy aging and cognitive booster activities in <i>C. elegans</i> and as well as in <i>M. musculus</i>. As such, the present herbal product thus developed may be quite useful for healthy aging and cognitive boosting activities, and more so during this covid-19 pandemic.</p><p><strong>Graphical abstract: </strong></p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s13237-022-00407-1.</p>","PeriodicalId":53176,"journal":{"name":"Nucleus (India)","volume":" ","pages":"303-320"},"PeriodicalIF":2.1000,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9649024/pdf/","citationCount":"2","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Nucleus (India)","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1007/s13237-022-00407-1","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2022/11/10 0:00:00","PubModel":"Epub","JCR":"Q4","JCRName":"CELL BIOLOGY","Score":null,"Total":0}
引用次数: 2
Abstract
The positive effect of herbal supplements on aging and age-related disorders has led to the evolution of natural curatives for remedial neurodegenerative diseases in humans. The advancement in aging is exceedingly linked to oxidative stress. Enhanced oxidative stress interrupts health of humans in various ways, necessitating to find stress alleviating herbal resources. Currently, minimal scientifically validated health and cognitive booster resources are available. Therefore, we explored the impact of plant extracts in different combinations on oxidative stress, life span and cognition using the multicellular transgenic humanized C. elegans, and further validated the same in Mus musculus, besides testing their safety and toxicity. In our investigations, the final product-the HACBF (healthy ageing cognitive booster formulation) thus developed was found to reduce major aging biomarkers like lipofuscin, protein carbonyl, lipid levels and enhanced activity of antioxidant enzymes. Further confirmation was done using transgenic worms and RT-PCR. The cognitive boosting activities analyzed in C. elegans and M. musculus model system were found to be at par with donepezil and L-dopa, the two drugs which are commonly used to treat Parkinson's and Alzheimer's diseases. In the transgenic C. elegans model system, the HACBF exhibited reduced aggregation of misfolded disease proteins α-synuclein and increased the health of nicotinic acetylcholine receptor, levels of Acetylcholine and Dopamine contents respectively, the major neurotransmitters responsible for memory, language, learning behavior and movement. Molecular studies clearly indicate that HACBF upregulated major genes responsible for healthy aging and cognitive booster activities in C. elegans and as well as in M. musculus. As such, the present herbal product thus developed may be quite useful for healthy aging and cognitive boosting activities, and more so during this covid-19 pandemic.
Graphical abstract:
Supplementary information: The online version contains supplementary material available at 10.1007/s13237-022-00407-1.
Nucleus (India)Biochemistry, Genetics and Molecular Biology-Genetics
CiteScore
3.90
自引率
0.00%
发文量
26
期刊介绍：
The journal publishes original, peer-reviewed articles on new and significant advances in all aspects of cell and chromosome research. Timely and substantial articles in the areas of cell biology, genetic toxicology, chromosome evolution, cytogenetics; molecular-, population- and evolutionary genetics; epigenetics; developmental and stress biology; transcriptomics, structural and functional genomics, proteomics, metabolomics, integrated omics and use of tools of bioinformatics in the areas stated herein. Studies demonstrating the use of modern approaches such as genome editing to address technological problems and/or biological questions pertaining to the research areas mentioned above are encouraged. However, the studies should be conducted with appropriate scientific rigor in the design, conduct, validity, and interpretation of results to ensure reliability and reproducibility of the data presented. The papers must be written concisely and be of interest to a broad audience.
The journal also publishes comprehensive reviews on current developments and future trends in cell and chromosome research. Such articles should be authoritative, covering all the aspects of a chosen topic, and serve as a resource for students, researchers, and multidisciplinary audience. Authors are welcome to contact the Editor-in-Chief to discuss their topic of interest while preparing a prospective review article.
Thematic special issues on cutting edge research in the development of the above stated areas are periodically published by the journal. Articles for such issues are commissioned by the respective topic editor/s.
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