Evaluation of the Biodistribution of Arginine, glycine, aspartic acid peptide-modified Nanoliposomes Containing Curcumin in Rats.

IF 1.6 4区生物学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Iranian Journal of Biotechnology Pub Date : 2022-04-01 DOI:10.30498/ijb.2021.272168.2990

Parisa Salehi Najafabadi, Hamdollah Delaviz, Arash Asfaram, Mehrzad Jafari Barmak, Amir Ghanbari, Mohsen Alipour, Parastou Rad, Hassan Bardania

{"title":"Evaluation of the Biodistribution of Arginine, glycine, aspartic acid peptide-modified Nanoliposomes Containing Curcumin in Rats.","authors":"Parisa Salehi Najafabadi, Hamdollah Delaviz, Arash Asfaram, Mehrzad Jafari Barmak, Amir Ghanbari, Mohsen Alipour, Parastou Rad, Hassan Bardania","doi":"10.30498/ijb.2021.272168.2990","DOIUrl":null,"url":null,"abstract":"Background: Liposomes, as a biological membrane, is successfully used for drug delivery, reduces toxicity in normal cells and improves bio-accessibility of the drug to the target cells. Curcumin, as a bioactive substance with pleiotropic biological activities, is an anti-inflammatory compound and has several anticancer effects in different cancers such as pancreatic and breast cancer.Objectives: This study was conducted to determine the bio-distribution of arginine-glycine-aspartic acid (RGD)-modified nanoliposomes containing curcumin in different tissues of rats.Materials and methods: The amount of curcumin in each tissue was examined by HPLC analysis. The distribution of liposomal Hoechst in the rats was evaluated by using fluorescence spectrophotometry, live animal imaging analyses and histological methods.Results: HPLC analysis showed the mean of curcumin in the blood significantly increased in the liposomal curcumin modified with RGD compared to free curcumin. These results were confirmed by fluorescence measurement for RGD modified liposome containing Hoechst dye. There was negligible fluorescent intensity in the blood rats, which received Hoechst alone. Live animal imaging analysis showed the presence of fluorescent color in heart tissue for all groups. It was also detected in kidney tissue for liposomal Hoechst modified with RGD group.Conclusions: The present study demonstrated that RGD-modified nano-liposomes can significantly improve drug retention time in the blood of rats.","PeriodicalId":14492,"journal":{"name":"Iranian Journal of Biotechnology","volume":null,"pages":null},"PeriodicalIF":1.6000,"publicationDate":"2022-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/61/56/IJB-20-e2990.PMC9583817.pdf","citationCount":"3","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Iranian Journal of Biotechnology","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.30498/ijb.2021.272168.2990","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}

引用次数: 3

Abstract

Background: Liposomes, as a biological membrane, is successfully used for drug delivery, reduces toxicity in normal cells and improves bio-accessibility of the drug to the target cells. Curcumin, as a bioactive substance with pleiotropic biological activities, is an anti-inflammatory compound and has several anticancer effects in different cancers such as pancreatic and breast cancer.

Objectives: This study was conducted to determine the bio-distribution of arginine-glycine-aspartic acid (RGD)-modified nanoliposomes containing curcumin in different tissues of rats.

Materials and methods: The amount of curcumin in each tissue was examined by HPLC analysis. The distribution of liposomal Hoechst in the rats was evaluated by using fluorescence spectrophotometry, live animal imaging analyses and histological methods.

Results: HPLC analysis showed the mean of curcumin in the blood significantly increased in the liposomal curcumin modified with RGD compared to free curcumin. These results were confirmed by fluorescence measurement for RGD modified liposome containing Hoechst dye. There was negligible fluorescent intensity in the blood rats, which received Hoechst alone. Live animal imaging analysis showed the presence of fluorescent color in heart tissue for all groups. It was also detected in kidney tissue for liposomal Hoechst modified with RGD group.

Conclusions: The present study demonstrated that RGD-modified nano-liposomes can significantly improve drug retention time in the blood of rats.

Abstract Image

查看原文本刊更多论文

精氨酸、甘氨酸、天冬氨酸肽修饰的姜黄素纳米脂质体在大鼠体内生物分布的评价。

背景:脂质体作为一种生物膜，成功地用于药物传递，降低了正常细胞中的毒性，提高了药物对靶细胞的生物可及性。姜黄素是一种具有多种生物活性的生物活性物质，是一种抗炎化合物，对胰腺癌、乳腺癌等不同的癌症具有多种抗癌作用。目的:研究含姜黄素的精氨酸-甘氨酸-天冬氨酸修饰纳米脂质体在大鼠不同组织中的生物分布。材料与方法:采用高效液相色谱法测定各组组织中姜黄素的含量。采用荧光分光光度法、活体显像法和组织学方法观察大鼠Hoechst脂质体的分布。结果:高效液相色谱分析显示，与游离姜黄素相比，经RGD修饰的姜黄素脂质体中血液中姜黄素的平均值明显升高。对含赫斯特染料的RGD修饰脂质体进行了荧光测定，证实了上述结果。仅接受赫斯特治疗的血鼠的荧光强度可以忽略不计。活体动物成像分析显示，所有组的心脏组织均存在荧光色。RGD修饰的Hoechst脂质体在肾组织中也有检测。结论:本研究表明，rgd修饰的纳米脂质体可以显著提高药物在大鼠血液中的滞留时间。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Iranian Journal of Biotechnology BIOTECHNOLOGY & APPLIED MICROBIOLOGY-

CiteScore

2.60

自引率

7.70%

发文量

期刊介绍： Iranian Journal of Biotechnology (IJB) is published quarterly by the National Institute of Genetic Engineering and Biotechnology. IJB publishes original scientific research papers in the broad area of Biotechnology such as, Agriculture, Animal and Marine Sciences, Basic Sciences, Bioinformatics, Biosafety and Bioethics, Environment, Industry and Mining and Medical Sciences.