Circulatory cytokeratin 17, marginal zone B1 protein and leucine-rich α2-glycoprotein-1 as biomarkers for disease severity and fibrosis in systemic sclerosis patients.

IF 3.8 3区 医学 Q1 MEDICAL LABORATORY TECHNOLOGY
Paul Bălănescu, Eugenia Bălănescu, Cristian Băicuș, Anca Bălănescu
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引用次数: 2

Abstract

Introduction: Systemic sclerosis (Ssc) is a multiorgan debilitating autoimmune disease that associates the triad: vascular involvement, tissue fibrosis and profound immune response alterations. Numerous previous studies focused on identification of candidate proteomic Ssc biomarkers using mass-spectrometry techniques and a large number of candidate Ssc biomarkers emerged. These biomarkers must firstly be confirmed in independent patient groups. The aim of the present study was to investigate the association of cytokeratin 17 (CK17), marginal zone B1 protein (MZB1) and leucine-rich α2-glycoprotein-1 (LRG1) with clinical and biological Ssc characteristics.

Material and methods: Serum CK17, MZB1 and LRG1 were assessed in samples of the available Ssc biobank comprising of samples from 53 Ssc patients and 26 matched age and gender controls.

Results: Circulatory CK17, LRG1 and MZB1 concentrations were increased in Ssc patients. Cytokeratin 17 is independently associated with Ssc disease activity. Patients with pulmonary fibrosis expressed higher LRG1 and MZB1 concentrations. Serum MZB1 concentrations were also associated with extensive skin fibrosis.

Conclusions: Serum CK17, MZB1 and LRG1 were confirmed biomarkers for Ssc. LRG1 seems a good biomarker for pulmonary fibrosis, while MZB1 is a good biomarker for extensive skin fibrosis. CK17 proved to be independently associated with Ssc disease severity, higher CK17 values being protective for a more active disease.

循环细胞角蛋白17、边缘区B1蛋白和富含亮氨酸的α2糖蛋白-1作为系统性硬化症患者疾病严重程度和纤维化的生物标志物
简介:系统性硬化症(Ssc)是一种多器官衰弱性自身免疫性疾病,与血管受累、组织纤维化和深刻的免疫反应改变三要素有关。以往的大量研究集中在利用质谱技术鉴定候选蛋白质组学Ssc生物标志物上,并出现了大量候选Ssc生物标志物。这些生物标志物必须首先在独立的患者群体中得到证实。本研究旨在探讨细胞角蛋白17 (CK17)、边缘区B1蛋白(MZB1)和富含亮氨酸的α2糖蛋白-1 (LRG1)与Ssc临床和生物学特征的关系。材料和方法:血清CK17, MZB1和LRG1在现有Ssc生物库样本中进行评估,该样本包括53名Ssc患者和26名年龄和性别匹配的对照组。结果:Ssc患者循环CK17、LRG1和MZB1浓度升高。细胞角蛋白17与Ssc疾病活动性独立相关。肺纤维化患者表达更高的LRG1和MZB1浓度。血清MZB1浓度也与广泛的皮肤纤维化有关。结论:血清CK17、MZB1和LRG1是Ssc的生物标志物。LRG1似乎是肺纤维化的良好生物标志物,而MZB1是广泛皮肤纤维化的良好生物标志物。CK17被证明与Ssc疾病严重程度独立相关,较高的CK17值对更活跃的疾病有保护作用。
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来源期刊
Biochemia Medica
Biochemia Medica 医学-医学实验技术
CiteScore
5.50
自引率
3.00%
发文量
70
审稿时长
>12 weeks
期刊介绍: Biochemia Medica is the official peer-reviewed journal of the Croatian Society of Medical Biochemistry and Laboratory Medicine. Journal provides a wide coverage of research in all aspects of clinical chemistry and laboratory medicine. Following categories fit into the scope of the Journal: general clinical chemistry, haematology and haemostasis, molecular diagnostics and endocrinology. Development, validation and verification of analytical techniques and methods applicable to clinical chemistry and laboratory medicine are welcome as well as studies dealing with laboratory organization, automation and quality control. Journal publishes on a regular basis educative preanalytical case reports (Preanalytical mysteries), articles dealing with applied biostatistics (Lessons in biostatistics) and research integrity (Research integrity corner).
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