Phthalates in albumin from human serum: implications for assisted reproductive technology

Jennifer R. Hughes Ph.D. , Sandra Soto-Heras Ph.D. , Charles H. Muller Ph.D. , David J. Miller Ph.D.
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引用次数: 1

Abstract

Albumin, a vital protein in cell culture systems, is derived from whole blood or blood products. The culture of human gametes and developing embryos for assisted reproductive technology (ART) uses albumin of human origin. Human serum albumin (HSA) is derived from expired blood obtained from blood banks. This blood has been stored in polyvinyl chloride bags made clear and flexible with di-2-ethylhexyl phthalate (DEHP). However, DEHP can leach from the bags into stored blood and cofractionate with HSA during albumin isolation. DEHP and its metabolite, mono-ethylhexyl phthalate, are known endocrine disruptors that are reported to have negative effects when directly supplemented in media for in vitro fertilization using gametes from a variety of animals. Therefore, the contamination of ART media with DEHP and mono-ethylhexyl phthalate through HSA supplementation may affect the outcomes of ART procedures. Although the embryology laboratory is strictly monitored to prevent a wide variety of contaminations, phthalate contamination of HSA has not been broadly examined. This review outlines the function of HSA in ART procedures and the production of HSA from whole blood. Finally, the review highlights the effects of acute phthalate exposures on gametes during in vitro procedures. Phthalates found in human serum albumin are present in media used for ART at levels that impair developmental endpoints in model species.

人血清白蛋白中的邻苯二甲酸酯:对辅助生殖技术的影响
白蛋白是细胞培养系统中的一种重要蛋白质,来源于全血或血液制品。用于辅助生殖技术(ART)的人类配子和发育胚胎的培养使用来自人类的白蛋白。人血清白蛋白(HSA)是从血库获得的过期血液中提取的。这些血液被储存在用邻苯二甲酸二乙己基酯(DEHP)制成的透明且柔软的聚氯乙烯袋中。然而,DEHP可以从袋子中渗透到储存的血液中,并在白蛋白分离期间与HSA分解。DEHP及其代谢物邻苯二甲酸单乙基己酯是已知的内分泌干扰物,据报道,在使用各种动物配子进行体外受精的培养基中直接补充DEHP会产生负面影响。因此,通过补充HSA使ART培养基被DEHP和邻苯二甲酸单乙基己酯污染可能会影响ART手术的结果。尽管胚胎学实验室受到严格监控,以防止各种污染,邻苯二甲酸盐污染的HSA尚未广泛检查。本文综述了HSA在抗逆转录病毒治疗过程中的作用以及从全血中产生HSA的情况。最后,回顾强调急性邻苯二甲酸盐暴露对配子在体外过程中的影响。在人血清白蛋白中发现的邻苯二甲酸酯存在于用于抗逆转录病毒治疗的培养基中,其水平会损害模型物种的发育终点。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
F&S reviews
F&S reviews Endocrinology, Diabetes and Metabolism, Obstetrics, Gynecology and Women's Health, Urology
CiteScore
3.70
自引率
0.00%
发文量
0
审稿时长
61 days
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