Seminal plasma extracellular vesicles tRF-Val-AAC-010 can serve as a predictive factor of successful microdissection testicular sperm extraction in patients with non-obstructive azoospermia.

Reproductive biology and endocrinology : RB&E Pub Date : 2022-07-22 DOI:10.1186/s12958-022-00978-3

Xiaoxiao Han, Lin Hao, Zhenduo Shi, Ying Li, Liang Wang, Zhenbei Li, Qiang Zhang, Fangfang Hu, Yijuan Cao, Kun Pang, Zuobin Zhu

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引用次数: 5

Abstract

Background: There is a lack of biomarkers for distinguishing non-obstructive azoospermia (NOA) patients with successful sperm retrieval (Sp+) from those with failed sperm retrieval (Sp-). This study aimed to determine the potential of extracellular vesicles tRNA-derived small RNA (tsRNA) as a novel non-invasive biomarker for successful sperm retrieval by microdissection testicular sperm extraction (mTESE).

Methods: The study included 18 patients with NOA with successful sperm retrieval (Sp+) and 23 patients with NOA with failed sperm retrieval (Sp-), 15 obstructive azoospermia (OA) patients, 5 idiopathic oligospermia (IO) patients, and 12 healthy people. Seminal plasma extracellular vesicles tsRNA levels were used in a two-stage case-control study (screened by tsRNA sequencing on Illumina NextSeq instrument and validated by qRT-PCR). The bioinformatic analysis was performed to determine the role of tsRNA in the pathogenesis of non-obstructive azoospermia.

Results: Two tsRNAs (tRF-Val-AAC-010: AUC = 0.96, specificity = 80%, sensitivity = 95%; tRF-Pro-AGG-003: AUC = 0.96, specificity = 87%, sensitivity = 95%) were found to have high predictive accuracy for distinguishing the origin of azoospermia. In addition, the extracellular vesicles tRF-Val-AAC-010 resulted in high predictive ability (AUC = 0.89, sensitivity = 72%, specificity = 91%, P < 0.0001) in predicting the presence of sperm in non-obstructive azoospermia undergoing mTESE. Finally, bioinformatic analysis revealed that tRF-Val-AAC-010 were involved in spermatogenesis.

Conclusions: This study identified that the extracellular vesicles tRF-Val-AAC-010 and tRF-Pro-AGG-003 are biomarkers for the diagnosis of non-obstructive azoospermia, and that tRF-Val-AAC-010 as a potential non-invasive biomarker for predicting the presence of sperm in non-obstructive azoospermia testicular tissue.

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精浆细胞外囊泡tRF-Val-AAC-010可作为非阻塞性无精子症患者显微解剖睾丸精子提取成功的预测因素。

背景:缺乏生物标志物来区分非阻塞性无精子症(NOA)患者的成功精子回收(Sp+)和那些失败的精子回收(Sp-)。本研究旨在确定细胞外小泡trna衍生小RNA (tsRNA)作为一种新的无创生物标志物的潜力，用于通过显微解剖睾丸精子提取(mTESE)成功提取精子。方法:18例NOA取精成功(Sp+)患者和23例NOA取精失败(Sp-)患者，15例阻塞性无精子症(OA)患者，5例特发性少精子症(IO)患者，12例健康人群。精浆细胞外囊泡tsRNA水平用于两期病例对照研究(通过Illumina NextSeq仪器上的tsRNA测序筛选，并通过qRT-PCR验证)。通过生物信息学分析确定tsRNA在非阻塞性无精子症发病机制中的作用。结果:2个tsRNAs (tRF-Val-AAC-010: AUC = 0.96，特异性= 80%，敏感性= 95%;发现tRF-Pro-AGG-003: AUC = 0.96，特异性= 87%，敏感性= 95%)对区分无精子症的来源具有较高的预测准确性。此外，细胞外囊泡tRF-Val-AAC-010具有较高的预测能力(AUC = 0.89，灵敏度= 72%，特异性= 91%)。结论:本研究发现细胞外囊泡tRF-Val-AAC-010和tRF-Pro-AGG-003是诊断非梗阻性无精子症的生物标志物，tRF-Val-AAC-010是预测非梗阻性无精子症睾丸组织中精子存在的潜在无创生物标志物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Reproductive biology and endocrinology : RB&E

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