[Gene Sequencing Analyses of 10 ABO Ambiguous Blood Group Samples].

Hui Ni Huang, Zhu Ning Mo, Xiang Cheng Liao, Zi Ji Yang, Yue Rong Wei, Chun Li Zhu, Hai Lan Li
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引用次数: 0

Abstract

Objective: Through analysis of ABO blood group gene typing technology, to assist in the identification of difficult clinical serological specimens.

Methods: A total of 10 forwardreverse typing ambiguous samples were collected from January 2021 to August 2021 in our hospital.ABO genotypes were analysed by gene sequencing.

Results: The genotypes of 10 ABO ambiguous blood group samples were A102/BW11, A102/BW12, O02/O02, A102/B303, A102/B101, BW11/O02, B101/O04, BW11/O01, BW11/O01, A101/O02, respectively. The genotype results of 6 cases was consistent with the serological phenotype, and the serological phenotype of 4 cases were different from the geno sequencing.

Conclusion: ABO blood groups genotyping technology combined with serological typing can be used for accurate typing of ambiguous blood group, and better ensure the safety of blood transfusion.

10份ABO歧义血型样本的基因测序分析
目的:通过对ABO血型基因分型技术的分析,辅助临床血清学标本鉴定。方法:于2021年1月至2021年8月在我院收集10例正向、反向分型模糊样本。采用基因测序法分析ABO基因型。结果:10份ABO歧见血型的基因型分别为A102/BW11、A102/BW12、O02/O02、A102/B303、A102/B101、BW11/O02、B101/O04、BW11/O01、BW11/O01、A101/O02。6例基因型结果与血清学表型一致,4例血清学表型与基因测序结果不一致。结论:ABO血型基因分型技术结合血清学分型可对歧义血型进行准确分型,更好地保证输血安全。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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