Primacy of Human Odors Over Visual and Heat Cues in Inducing Landing in Female Aedes aegypti Mosquitoes.

Abstract

Although human skin odor is thought to be the cue that anthropophilic mosquitoes use to discriminate us from other potential hosts, the precise details of how they use skin odor to find and land on a human is unclear. We found that Aedes aegypti land on a source of skin odor without a co-located visual cue. By collecting human odor on glass beads and using identical glass beads to visually conceal skin odor and heat cues, we were able to study mosquito landing on skin odor, heat, and visual cues separately. Landing is necessary for blood feeding which is a required behavior for the Aedes aegypti life cycle as well as the behavior responsible for the epidemiological impact of mosquitoes. Therefore, we consider it to be the diagnostic measure of the importance of a host cue. In two-choice tests, a skin odor source had the highest valence for landing, followed by a combination of heat and a visual cue, and finally heat and visual cues presented separately. We also measured the durations of the landings, though no significant differences were found.

Supplementary information: The online version contains supplementary material available at 10.1007/s10905-022-09796-2.