Evaluation of Matrix-assisted laser desorption/ionization Time-of flight Mass spectrometry (MALDI TOF MS) and VITEK 2 in routine microbial identification.

Q3 Medicine

Ghana Medical Journal Pub Date : 2021-12-01 DOI:10.4314/gmj.v55i4.12

Anitha Madhavan, Arun Sachu, Nandini Sethuraman, Anil Kumar, Jayalakshmi Vasudevapanicker

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引用次数: 0

Abstract

Bacground: Microbial Identification was done by phenotypic methods. VITEK-2 and Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) are now being increasingly used in laboratories.

Objectives: To compare and evaluate the usefulness of MALDI-TOF MS and VITEK-2 in routine microbial identification.

Methods: The performances of MALDI-TOF MS and VITEK 2 were compared for identifying microorganisms.

Results: MALDI- TOF MS and VITEK-2 correctly identified 96 % (96/100) and 97% (97/100) of the isolates upto the genus level.

Conclusion: MALDI TOF MS and VITEK -2 gave comparable identification and error rates. The rapid reduction in turnaround time with MALDI TOF is a significant game-changer in the field of clinical microbiology.

Funding: State Board of Medical Research (SBMR).

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基质辅助激光解吸/电离飞行时间质谱(MALDI TOF MS)和VITEK 2在常规微生物鉴定中的评价。

背景:微生物鉴定采用表型法。VITEK-2和基质辅助激光解吸电离飞行时间质谱(MALDI-TOF MS)现在越来越多地用于实验室。目的:比较和评价MALDI-TOF MS和VITEK-2在常规微生物鉴定中的应用价值。方法:比较MALDI-TOF MS和VITEK 2鉴别微生物的性能。结果:MALDI- TOF MS和VITEK-2鉴别正确率分别为96%(96/100)和97%(97/100)。结论:MALDI TOF MS与VITEK -2鉴别错误率相当。快速减少周转时间与MALDI TOF是一个重要的游戏规则改变在临床微生物学领域。资助:国家医学研究委员会(SBMR)。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Ghana Medical Journal Medicine-Medicine (all)

CiteScore

1.10

自引率

0.00%

发文量

审稿时长

20 weeks