Acrylamide-based hydrogels with distinct osteogenic and chondrogenic differentiation potential.

IF 4.4 3区 医学 Q2 ENGINEERING, BIOMEDICAL
Progress in Biomaterials Pub Date : 2022-09-01 Epub Date: 2022-07-16 DOI:10.1007/s40204-022-00196-5
Z M Younus, P Roach, N R Forsyth
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引用次数: 1

Abstract

Regeneration solutions for the osteochondral interface depth are limited, where multi-material implants have the potential to delaminate affecting the regeneration process and impacting the final integrity of tissue interface. Here we explore regionally mixed hydrogel networks, presenting distinct chemical features to determine their compatibility in supporting osteogenic or chondrogenic cell behaviour and differentiation. Poly(N-isopropylacrylamide) (pNIPAM) and poly(N-tert-butylacrylamide) (pNTBAM) hydrogels were assessed in terms of their chemical differences, mechanical strength, internal architecture, porosity and capacity to support cell viability, migration, and differentiation. pNTBAM polymerized with a Young's modulus of up to 371 ± 31 kPa compared to the more flexible pNIPAM, 16.5 ± 0.6 kPa. Viability testing revealed biocompatibility of both hydrogels with significantly increased cell numbers observed in pNTBAM (500 ± 95 viable cells/mm2) than in pNIPAM (60 ± 3 viable cells/mm2) (P ≤ 0.05). Mineralization determined through alkaline phosphatase (ALP) activity, calcium ion and annexin A2 markers of mineralization) and osteogenic behaviour (collagen I expression) were supported in both hydrogels, but to a greater extent in pNTBAM. pNTBAM supported significantly elevated levels of chondrogenic markers as evidenced by collagen II and glycosaminoglycan expression in comparison to little or no evidence in pNIPAM (P ≤ 0.05). In conclusion, structurally similar, chemically distinct, acrylamide hydrogels display variable capacities in supporting osteochondral cell behaviours. These systems demonstrate spatial control of cell interaction through simple changes in monomer chemistry. Fine control over chemical presentation during the fabrication of biomaterial implants could lead to greater efficacy and targeted regeneration of semi-complex tissues.

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丙烯酰胺基水凝胶具有明显的成骨和软骨分化潜力。
骨软骨界面深度的再生解决方案是有限的,其中多材料植入物有可能分层影响再生过程并影响组织界面的最终完整性。在这里,我们探索区域混合的水凝胶网络,呈现出不同的化学特征,以确定它们在支持成骨或软骨细胞行为和分化方面的兼容性。对聚n -异丙基丙烯酰胺(pNIPAM)和聚n -叔丁基丙烯酰胺(pNTBAM)水凝胶的化学差异、机械强度、内部结构、孔隙度和支持细胞活力、迁移和分化的能力进行了评估。pNTBAM聚合后的杨氏模量高达371±31 kPa,而更灵活的pNIPAM为16.5±0.6 kPa。活性测试显示,两种水凝胶的生物相容性均显著提高,pNTBAM(500±95个活细胞/mm2)比pNIPAM(60±3个活细胞/mm2)的细胞数量显著增加(P≤0.05)。矿化(通过碱性磷酸酶(ALP)活性、钙离子和膜联蛋白A2矿化标志物)和成骨行为(I型胶原表达)在两种水凝胶中都得到支持,但在pNTBAM中更大程度上得到支持。与pNIPAM相比,pNTBAM支持显著升高的软骨生成标志物水平,胶原II和糖胺聚糖的表达证明了这一点(P≤0.05)。总之,结构相似,化学性质不同,丙烯酰胺水凝胶在支持骨软骨细胞行为方面表现出不同的能力。这些系统通过单体化学的简单变化证明了细胞相互作用的空间控制。在生物材料植入物的制造过程中,对化学物质的精细控制可以提高半复杂组织的效率和靶向再生。
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来源期刊
Progress in Biomaterials
Progress in Biomaterials MATERIALS SCIENCE, BIOMATERIALS-
CiteScore
9.60
自引率
4.10%
发文量
35
期刊介绍: Progress in Biomaterials is a multidisciplinary, English-language publication of original contributions and reviews concerning studies of the preparation, performance and evaluation of biomaterials; the chemical, physical, biological and mechanical behavior of materials both in vitro and in vivo in areas such as tissue engineering and regenerative medicine, drug delivery and implants where biomaterials play a significant role. Including all areas of: design; preparation; performance and evaluation of nano- and biomaterials in tissue engineering; drug delivery systems; regenerative medicine; implantable medical devices; interaction of cells/stem cells on biomaterials and related applications.
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