Comprehensive Analysis on the Specific Role and Function of Mitochondrial Inner Membrane Protein MPV17 in Liver Hepatocellular Carcinoma.

IF 1.4 4区生物学 Q4 GENETICS & HEREDITY

Genetics research Pub Date : 2022-07-19 eCollection Date: 2022-01-01 DOI:10.1155/2022/7236823

Hanchuan Tao, Cheng Wang, Chongmei Lu, Ning Ma, Yifan Zhu, Shihai Xuan, Xiaojun Zhou

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引用次数: 1

Abstract

Background: Liver hepatocellular carcinoma (LIHC) is the predominant type of liver cancer, and its treatment still faces great challenges presently. Mitochondrial inner membrane protein MPV17 is reported to be involved in multiple biological activities of cancers. Here, we seek to investigate the specific role and functions of MPV17 in LIHC progression.

Methods: Firstly, MPV17 expressions in various tumors and corresponding normal samples and LIHC groups with various clinical features were analyzed, respectively. Next, the relationship between MPV17 expression and LIHC survival was analyzed and verified by AUC curves. Besides, differentially expressed genes (DEGs) for LIHC were screened from TCGA and then analyzed by GO and KEGG. Then, MPV17 was analyzed by prognostic model, Cox analysis, predictive nomogram, pathway correlation, and immunoassay. Finally, the functions of MPV17 were determined by CCK-8 and Tranwell assays.

Results: In most tumors, MPV17 expression was higher than that in the normal group, and it was related to LIHC clinical features. In the LIHC survival analysis, highly expressed MPV17 was associated with a poor prognosis. Besides, 314 upregulated and 193 downregulated DEGs are mainly involved in the TNF signaling pathway and tyrosine metabolism. Through prognostic model, Cox analysis, and predictive nomogram, MPV17 had the prognostic value for LIHC. Gene-pathway correlation analysis showed that MPV17 had the strongest correlation with the G2M_checkpoint pathway. In an immunoassay, MPV17 had a strong correlation with many immune cells. Functional assays showed that MPV17 reduction in LIHC cells could inhibit cell invasion, migration, and proliferation.

Conclusion: MPV17, as a tumor promoter, could be a new biomarker for LIHC diagnosis and prognosis and probably shed new light on the exploration of LIHC therapies.

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线粒体内膜蛋白MPV17在肝癌中的特殊作用和功能的综合分析。

背景:肝细胞癌(LIHC)是肝癌的主要类型，目前其治疗仍面临很大挑战。线粒体内膜蛋白MPV17被报道参与多种癌症的生物学活动。在这里，我们试图研究MPV17在LIHC进展中的具体作用和功能。方法:首先分析MPV17在不同肿瘤及相应的正常样本和不同临床特征的LIHC组中的表达情况。接下来，分析MPV17表达与LIHC存活的关系，并通过AUC曲线进行验证。此外，从TCGA中筛选LIHC的差异表达基因(DEGs)，然后通过GO和KEGG进行分析。然后通过预后模型、Cox分析、预测nomogram、pathway correlation和immunoassay对MPV17进行分析。最后用CCK-8和Tranwell法检测MPV17的功能。结果:MPV17在大多数肿瘤中表达均高于正常组，且与LIHC临床特征有关。在LIHC生存分析中，高表达MPV17与不良预后相关。此外，314个上调的deg和193个下调的deg主要参与TNF信号通路和酪氨酸代谢。通过预后模型、Cox分析和预测nomogram分析，MPV17对LIHC具有预测价值。基因通路相关分析显示MPV17与G2M_checkpoint通路相关性最强。在免疫分析中，MPV17与许多免疫细胞有很强的相关性。功能实验表明，MPV17的减少可以抑制LIHC细胞的侵袭、迁移和增殖。结论:MPV17作为一种肿瘤启动子，可能成为LIHC诊断和预后的新生物标志物，并可能为LIHC治疗的探索提供新的思路。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Genetics research 生物-遗传学

自引率

6.70%

发文量

审稿时长

>12 weeks

期刊介绍： Genetics Research is a key forum for original research on all aspects of human and animal genetics, reporting key findings on genomes, genes, mutations and molecular interactions, extending out to developmental, evolutionary, and population genetics as well as ethical, legal and social aspects. Our aim is to lead to a better understanding of genetic processes in health and disease. The journal focuses on the use of new technologies, such as next generation sequencing together with bioinformatics analysis, to produce increasingly detailed views of how genes function in tissues and how these genes perform, individually or collectively, in normal development and disease aetiology. The journal publishes original work, review articles, short papers, computational studies, and novel methods and techniques in research covering humans and well-established genetic organisms. Key subject areas include medical genetics, genomics, human evolutionary and population genetics, bioinformatics, genetics of complex traits, molecular and developmental genetics, Evo-Devo, quantitative and statistical genetics, behavioural genetics and environmental genetics. The breadth and quality of research make the journal an invaluable resource for medical geneticists, molecular biologists, bioinformaticians and researchers involved in genetic basis of diseases, evolutionary and developmental studies.