Enriched tandem repeats in chromosomal fusion points of Rineloricaria latirostris (Boulenger, 1900) (Siluriformes: Loricariidae).

IF 2.3 3区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Genome Pub Date : 2022-09-01 Epub Date: 2022-08-08 DOI:10.1139/gen-2022-0043
Larissa Glugoski, Viviane Nogaroto, Geize Aparecida Deon, Matheus Azambuja, Orlando Moreira-Filho, Marcelo Ricardo Vicari
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引用次数: 4

Abstract

Cytogenetic data showed the enrichment of repetitive DNAs in chromosomal rearrangement points between closely related species in armored catfishes. Still, few studies integrated cytogenetic and genomic data aiming to identify their prone-to-break DNA sites. Here, we aimed to obtain the repetitive fraction in Rineloricaria latirostris to recognize the microsatellite and homopolymers flanking the regions previously described as chromosomal fusion points. The results indicated that repetitive DNAs in R. latirostris are predominantly DNA transposons, and considering the microsatellite and homopolymers, A/T-rich expansions were the most abundant. The in situ localization demonstrated the A/T-rich repetitive sequences were scattered on the chromosomes, while A/G-rich microsatellite units were accumulated in some regions. The DNA transposon hAT, the 5S rDNA, and 45S rDNA (previously identified in Robertsonian fusion points in R. latirostris) were clusterized with some microsatellites, especially (CA)n, (GA)n, and poly-A, which were also enriched in regions of chromosomal fusions. Our findings demonstrated that repetitive sequences such as rDNAs, hAT transposons, and microsatellite units flank probable evolutionary breakpoint regions in R. latirostris. However, due to the sequence unit homologies in different chromosomal sites, these repeat DNAs only may facilitate chromosome fusion events in R. latirostris rather than working as a double-strand breakpoint site.

在染色体融合点上富集串联重复序列(Boulenger, 1900)(蛭形目:蛭形螨科)。
细胞遗传学数据显示,在亲缘关系密切的甲鱼中,重复dna在染色体重排点上富集。然而,很少有研究将细胞遗传学和基因组数据结合起来,旨在确定它们的前断裂DNA位点。在这里,我们的目的是获得重复分数,以识别微卫星和同聚物两侧的区域以前被描述为染色体融合点。结果表明,红豆豆的重复DNA以DNA转座子为主,考虑到微卫星和均聚物,A/ t富集扩增最为丰富。原位定位结果表明,富含A/ t的重复序列分散在染色体上,而富含A/ g的微卫星单元在某些区域积累。DNA转座子hAT、5S rDNA和45S rDNA(先前在r.l latirostris的Robertsonian融合点中发现)聚集了一些微卫星,特别是(CA)n、(GA)n和poly-A,这些微卫星也富集在染色体融合区域。我们的研究结果表明,rnas、hAT转座子和微卫星单元等重复序列位于可能的进化断点区域的侧面。然而,由于不同染色体位点的序列单位同源性,这些重复dna只可能促进染色体融合事件,而不是作为双链断点位点。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Genome
Genome 生物-生物工程与应用微生物
CiteScore
5.30
自引率
3.20%
发文量
42
审稿时长
6-12 weeks
期刊介绍: Genome is a monthly journal, established in 1959, that publishes original research articles, reviews, mini-reviews, current opinions, and commentaries. Areas of interest include general genetics and genomics, cytogenetics, molecular and evolutionary genetics, developmental genetics, population genetics, phylogenomics, molecular identification, as well as emerging areas such as ecological, comparative, and functional genomics.
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