Development and validation of a methodology for quantitative determination of malondialdehyde by HPLC-MC/MS.

Q4 Health Professions

Klinichescheskaya Laboratornaya Diagnostika Pub Date : 2022-07-18 DOI:10.51620/0869-2084-2022-67-7-369-373

P Yu Mylnikov, A V Shchulkin, Yuliya Vladimirovna Abalenikhina, E N Yakusheva

引用次数: 1

Abstract

A bioanalytical technique for quantitative determination of MDA by HPLC-MS/MS. The proposed method for determining MDA includes the release stage of bound MDA and excludes the derivatization reaction. The lower limit of quantitative detection was 600 nmol/l, the volume of the required sample was 10 µl, the analysis time was 7 min. The range of concentrations obtained during the study makes it possible to use this bioanalytical technique to determine the concentration of MDA in biological material when assessing physiological and pathological conditions.

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HPLC-MC/MS定量测定丙二醛方法的建立与验证。

HPLC-MS/MS定量测定丙二醛的生物分析技术。所提出的测定MDA的方法包括结合MDA的释放阶段，不包括衍生化反应。定量检测下限为600 nmol/l，所需样品体积为10µl，分析时间为7 min。在研究过程中获得的浓度范围使得可以使用该生物分析技术在评估生理和病理状况时确定生物材料中的MDA浓度。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Klinichescheskaya Laboratornaya Diagnostika Health Professions-Medical Laboratory Technology

CiteScore

0.90

自引率

0.00%

发文量

110

期刊介绍： The journal deals with theoretical and practical problems of clinical laboratory diagnosis, publishes editorial articles, reviews of literature, original articles, short reports, discussions, book reviews, current events, materials which may assist the practitioners, methods of laboratory investigations used in medicine, materials on the results of practical application of new methods of investigation in the following fields of clinical laboratory diagnosis: hematology, cytology, coagulation, biochemistry, immunology.