Signal amplification of araC pBAD using a standardized translation initiation region.

IF 2.6 Q2 BIOCHEMICAL RESEARCH METHODS

Synthetic biology (Oxford, England) Pub Date : 2022-07-05 eCollection Date: 2022-01-01 DOI:10.1093/synbio/ysac009

Patrick J Shilling, Diana Khananisho, Alister J Cumming, Bill Söderström, Daniel O Daley

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引用次数: 2

Abstract

araC pBAD is a genetic fragment that regulates the expression of the araBAD operon in bacteria, which is required for the metabolism of L-arabinose. It is widely used in bioengineering applications because it can drive regulatable and titratable expression of genes and genetic pathways in microbial cell factories. A notable limitation of araC pBAD is that it generates a low signal when induced with high concentrations of L-arabinose (the maximum ON state). Herein we have amplified the maximum ON state of araC pBAD by coupling it to a synthetically evolved translation initiation region (TIR^EVOL ). The coupling maintains regulatable and titratable expression from araC pBAD and yet increases the maximal ON state by >5-fold. The general principle demonstrated in the study can be applied to amplify the signal from similar genetic modules. Graphical Abstract.

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使用标准化翻译起始区域的araC pBAD信号放大。

araC pBAD是调节细菌中araBAD操纵子表达的基因片段，是l -阿拉伯糖代谢所必需的。它可以驱动微生物细胞工厂中可调节和可滴定的基因表达和遗传途径，因此在生物工程应用中得到广泛应用。araC pBAD的一个显著局限性是，高浓度l -阿拉伯糖(最大ON状态)诱导时产生低信号。本文通过将araC pBAD与合成进化的翻译起始区(TIREVOL)耦合，放大了araC pBAD的最大ON状态。这种耦合维持了araC pBAD的可调节和可滴定表达，但使最大ON状态增加了5倍以上。研究中证明的一般原理可以应用于放大来自类似遗传模块的信号。图形抽象。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Synthetic biology (Oxford, England)

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