Venom composition of Trimeresurus albolabris, T. insularis, T. puniceus and T. purpureomaculatus from Indonesia.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2022-07-06 eCollection Date: 2022-01-01 DOI:10.1590/1678-9199-JVATITD-2021-0103

Syahfitri Anita, Arif Rahman Sadjuri, Latri Rahmah, Herjuno Ari Nugroho, Mulyadi, Wahyu Trilaksono, Wiwit Ridhani, Nabila Safira, Hariman Bahtiar, Maharani, Amir Hamidy, Adriansjah Azhari

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引用次数: 4

Abstract

Background: Several studies have been published on the characterization of Trimeresurus venoms. However, there is still limited information concerning the venom composition of Trimeresurus species distributed throughout Indonesia, which contributes to significant snakebite envenomation cases. The present study describes a comparative on the composition of T. albolabris, T. insularis, T. puniceus, and T. purpureomaculatus venoms originated from Indonesia.

Methods: Protein content in the venom of four Trimeresurus species was determined using Bradford assay, and the venom proteome was elucidated using one-dimension SDS PAGE nano-ESI- LCMS/MS shotgun proteomics.

Results: The venom of T. albolabris contained the highest protein content of 11.1 mg/mL, followed by T. puniceus, T. insularis and T. purpureomaculatus venom with 10.7 mg/mL, 8.9 mg/mL and 5.54 mg/mL protein, respectively. In total, our venomic analysis identified 65 proteins belonging to 16 protein families in T. purpureomaculatus; 64 proteins belonging to 18 protein families in T. albolabris; 58 different proteins belonging to 14 protein families in T. puniceus; and 48 different proteins belonging to 14 protein familiesin T. insularis. Four major proteins identified in all venoms belonged to snake venom metalloproteinase, C-type lectin, snake venom serine protease, and phospholipase A2. There were 11 common proteins in all venoms, and T. puniceus venom has the highest number of unique proteins compared to the other three venoms. Cluster analysis of the proteins and venoms showed that T. puniceus venom has the most distinct venom composition.

Conclusions: Overall, the results highlighted venom compositional variation of four Trimeresurus spp. from Indonesia. The venoms appear to be highly similar, comprising at least four protein families that correlate with venom's toxin properties and function. This study adds more information on venom variability among Trimeresurus species within the close geographic origin and may contribute to the development of optimum heterologous antivenom.

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印度尼西亚大斑蝶、岛斑蝶、尖斑蝶和紫斑蝶的毒液组成。

背景:已经发表了一些关于Trimeresurus毒液特性的研究。然而，关于分布在印度尼西亚各地的Trimeresurus物种的毒液成分的信息仍然有限，这有助于显著的蛇咬中毒病例。本文对产自印度尼西亚的白斑绦虫(T. albolabris)、岛形绦虫(T. insularis)、尖形绦虫(T. puniceus)和紫斑绦虫(T. purpureomaculatus)毒液的组成进行了比较。方法:采用Bradford法测定4种三齿鲨毒液中的蛋白质含量，并采用一维SDS PAGE纳米esi - LCMS/MS霰弹枪蛋白质组学技术对其进行蛋白质组学分析。结果:黄颡鱼毒液中蛋白质含量最高，为11.1 mg/mL，其次为蛇颡鱼、岛颡鱼和紫颡鱼，蛋白质含量分别为10.7 mg/mL、8.9 mg/mL和5.54 mg/mL。总体而言，我们的毒理学分析鉴定了T. purpureomaculatus中16个蛋白家族的65个蛋白;红血球属18个蛋白家族的64个蛋白;水蛭属14个蛋白科58种不同蛋白;岛蝗中有14个蛋白质家族的48种不同蛋白质。在所有毒液中鉴定出的4种主要蛋白分别为蛇毒金属蛋白酶、c型凝集素、蛇毒丝氨酸蛋白酶和磷脂酶A2。所有毒液中都有11种常见蛋白质，与其他三种毒液相比，T. puniceus毒液中独特蛋白质的数量最多。蛋白质和毒液的聚类分析表明，水蛭毒液具有最明显的毒液成分。结论:总体而言，结果突出了印度尼西亚四种Trimeresurus属毒液成分的差异。毒液似乎高度相似，包括至少四个与毒液的毒素特性和功能相关的蛋白质家族。本研究进一步揭示了地理来源相近的Trimeresurus物种间毒液变异的信息，并有助于开发最佳的异源抗蛇毒血清。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.