Copaiba Oil Resin Exerts an Additive Effect to Babassu Oil on Behavioral Changes in Human Endometriotic Cell Cultures.

Pharmaceuticals (Basel, Switzerland) Pub Date : 2022-11-15 DOI:10.3390/ph15111414

Julianna Henriques da Silva, Leticia Coli Louvisse de Abreu, Renato Ferrari, Celia Yelimar Palmero Quintana, Eliane Gouvêa de Oliveira Barros, Natália de Moraes Cordeiro, Bruno Pontes, Valeria Pereira de Sousa, Lucio Mendes Cabral, Patricia Dias Fernandes, Luiz Eurico Nasciutti

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引用次数: 1

Abstract

Background: Current drugs for the treatment of endometriosis are not able to completely cure the condition, and significant side effects hinder the continuation of treatment. Therefore, it is necessary to search for new drug candidates. In the present paper, the use of plant extracts is highlighted. Babassu oil and Copaiba oil resin have several therapeutic properties. We investigated the in vitro effects of two nanoemulsions containing oil extracted from Babassu (Orbignya speciosa) nuts (called SNEDDS-18) and/or oil resin extracted from Copaiba trunk (Copaifera langsdorffii) (called SNEDDS-18/COPA) on cultured human eutopic endometrium stromal cells from endometrial biopsies of patients without (CESC) and with (EuESC) endometriosis as well as human stromal cells from biopsies of endometriotic lesions (EctESC).

Methods: CESC, EuESC, and EctESC were taken and treated with SNEDDS-18 and SNEDDS-18/COPA to evaluate their effects on cytotoxicity, cell morphology, proliferation, and signaling pathways.

Results: After 48 h of incubation with SNEDDS-18 and SNEDDS-18/COPA, cell viability and proliferation were inhibited, especially in EctESC. The lowest concentration of both nanoemulsions reduced cell viability and proliferation and broke down the cytoskeleton in EctESCs. After 24 h of treatment a decrease in IL-1, TNF-α, and MCP-1 was observed, as well as an increase in IL-10 production.

Conclusions: Both nanoemulsions can affect endometriotic stromal cell behaviors, thus revealing two potential candidates for new phytotherapeutic agents for the management of endometriosis.

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可可巴油树脂对巴巴苏油对人子宫内膜异位症细胞培养行为改变的加性作用。

背景:目前治疗子宫内膜异位症的药物并不能完全治愈这种疾病，而且严重的副作用阻碍了治疗的继续。因此，有必要寻找新的候选药物。本文重点介绍了植物提取物的应用。巴巴苏油和Copaiba油树脂具有几种治疗特性。我们研究了两种含有巴巴苏(Orbignya speciosa)坚果提取油(称为SNEDDS-18)和/或从Copaiba树干(Copaifera langsdorffii)提取油树脂(称为SNEDDS-18/COPA)的纳米乳液对体外培养的人异位子宫内膜基质细胞的影响，这些细胞来自于非(CESC)和(EuESC)子宫内膜异位症患者的子宫内膜活检，以及子宫内膜异位症病变活检(EctESC)的人基质细胞。方法:采用SNEDDS-18和SNEDDS-18/COPA处理CESC、EuESC和EctESC，观察其对细胞毒性、细胞形态、增殖和信号通路的影响。结果:SNEDDS-18和SNEDDS-18/COPA孵育48 h后，细胞活力和增殖均受到抑制，尤其是在EctESC中。在EctESCs中，两种纳米乳液的浓度均较低，降低了细胞活力和增殖，破坏了细胞骨架。处理24小时后，观察到IL-1、TNF-α和MCP-1的减少，IL-10的产生增加。结论:这两种纳米乳剂都能影响子宫内膜异位症基质细胞的行为，从而揭示了两种潜在的治疗子宫内膜异位症的植物药物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Pharmaceuticals (Basel, Switzerland)

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