Clinical Prospective Assessment of Genotoxic Effects of Dental Implants in Gingival Epithelial Cells.

IF 1.4 Q3 DENTISTRY, ORAL SURGERY & MEDICINE

Acta Stomatologica Croatica Pub Date : 2022-09-01 DOI:10.15644/asc56/3/1

Mirna Radovic, Lidia Gavic, Daniel Jerkovic, Davor Zeljezic, Jasna Puizina, Ivan Srzentic, Ema Puizina Mladinic, Antonija Tadin

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引用次数: 2

Abstract

Objectives: Although titanium-based implants are considered bioinert, it has been found that they are subject to corrosion and wear. This study aimed to evaluate the cytotoxic and genotoxic potential of two implant systems in gingival epithelial cells.

Material and methods: Gingival swabs were taken three times from 91 subjects. The first swab was taken before dental implant placement, the second swab 90 days after dental implant placement and the third swab 21 days following the healing abutment placement. DNA damage was analyzed using the micronucleus test. Tested dental implants with corresponding healing abutments were Ankylos and Dentium SuperLine.

Results: Of all scored forms of cytogenetic damage in gingival cells of individuals after implementation of tested dental implant systems, only an increase in the number of binucleated cells (P ≤ 0.001) was significant in contrast to control values for both tested implant systems, 90 days after dental implant placement and 21 days following the healing abutment placement.

Conclusion: It may be concluded that there are no titanium-based implant dependent cytogenetic damage in gingival epithelial cells. A slight increase in cytogenetic damage has been observed but it is of no biological relevance and might be associated with healing abutment induced effect.

Abstract Image

查看原文本刊更多论文

牙种植体对牙龈上皮细胞基因毒性作用的临床前瞻性评估。

虽然钛基植入物被认为是生物惰性的，但已经发现它们容易腐蚀和磨损。本研究旨在评估两种种植体系统对牙龈上皮细胞的细胞毒性和基因毒性。材料与方法:对91例受试者进行3次牙龈拭子采集。第一次拭子在种植体放置前采集，第二次拭子在种植体放置后90天采集，第三次拭子在基牙放置愈合后21天采集。用微核试验分析DNA损伤。测试的牙种植体与相应的愈合基台是Ankylos和Dentium SuperLine。结果:在实施测试的牙种植系统后，个体牙龈细胞中所有细胞遗传学损伤的评分形式中，只有双核细胞的数量增加(P≤0.001)，与两种测试的种植系统的控制值相比，在牙种植放置后90天和在愈合的基台放置后21天。结论:钛基种植体对牙龈上皮细胞无依赖性细胞遗传学损伤。已观察到细胞遗传学损伤的轻微增加，但它与生物学无关，可能与愈合基台诱导作用有关。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Acta Stomatologica Croatica DENTISTRY, ORAL SURGERY & MEDICINE-

CiteScore

2.40

自引率

28.60%

发文量

审稿时长

12 weeks

期刊介绍： The Acta Stomatologica Croatica (ASCRO) is a leading scientific non-profit journal in the field of dental, oral and cranio-facial sciences during the past 44 years in Croatia. ASCRO publishes original scientific and clinical papers, preliminary communications, case reports, book reviews, letters to the editor and news. Review articles are published by invitation from the Editor-in-Chief by acclaimed professionals in distinct fields of dental medicine. All manuscripts are subjected to peer review process.