Analysis of the relationship between the expression of EBV-related antibodies and ET-1 axis in gastric cancer.

IF 1.9
Yan Zhang, Qianqian Zhang, Lin Xu, Weiwen Wang, Hua Xiao, Bing Luo
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Abstract

Background and objective: EBV-associated gastric cancer (EBVaGC) is a distinct subtype of GC, and EBV plays an important role in tumor progress. The standard method to identify EBV-positive tumor is determined by in situ hybridization for EBV-encoded EBERs in tumor tissues. The present study aims to detect the serological expression of EBV-related antibodies and ET-1 axis to provide a noninvasive method for diagnosis of EBVaGC.

Methods: The content of EBV-related antibodies and ET-1 axis in preoperative peripheral blood of GC was performed by Chemiluminescence and ELISA assay. The EBV DNA copy number was measured by qRT-PCR.

Results: The results showed that the levels of anti-EBV early antigen (EA) IgG, viral capsid antigen (VCA) IgA, nuclear antigen (NA) IgG, and EBV DNA copy number were significantly higher in EBVaGC. The ET-1 axis level was much lower in EBVaGC than EBVnGC.

Conclusions: The combined detection of specific anti-EBV antibodies and ET-1 axis might provide new molecular markers for the identification of EBVaGC.

胃癌中ebv相关抗体表达与ET-1轴的关系分析。
背景与目的:EBV相关胃癌(EBVaGC)是胃癌的一种独特亚型,EBV在肿瘤进展中起重要作用。鉴定ebv阳性肿瘤的标准方法是对肿瘤组织中ebv编码的EBERs进行原位杂交。本研究旨在检测ebv相关抗体及ET-1轴的血清学表达,为EBVaGC的无创诊断提供一种方法。方法:采用化学发光法和ELISA法检测胃癌术前外周血ebv相关抗体及ET-1轴的含量。采用qRT-PCR检测EBV DNA拷贝数。结果:EBVaGC中抗EBV早期抗原(EA) IgG、病毒衣壳抗原(VCA) IgA、核抗原(NA) IgG水平和EBV DNA拷贝数均显著升高。EBVaGC的ET-1轴水平明显低于EBVnGC。结论:特异性抗ebv抗体与ET-1轴联合检测可能为EBVaGC的鉴定提供新的分子标记。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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