ANTIPROLIFERATIVE ACTIVITIES Of EXTRACTS FROM MYCELIAL BIOMASS OF SOME MEDICINAL BASIDIOMYCETES IN HUMAN COLON CANCER CELLS COLO 205.

Q3 Medicine

Experimental oncology Pub Date : 2022-11-01 DOI:10.32471/exp-oncology.2312-8852.vol-44-no-3.18434

L V Garmanchuk, N P Vedenicheva, G A Al-Maali, D I Ostapchenko, Yu V Tseyslyer, V A Liashenko, N A Bisko, I V Kosakivska, L I Ostapchenko

{"title":"ANTIPROLIFERATIVE ACTIVITIES Of EXTRACTS FROM MYCELIAL BIOMASS OF SOME MEDICINAL BASIDIOMYCETES IN HUMAN COLON CANCER CELLS COLO 205.","authors":"L V Garmanchuk, N P Vedenicheva, G A Al-Maali, D I Ostapchenko, Yu V Tseyslyer, V A Liashenko, N A Bisko, I V Kosakivska, L I Ostapchenko","doi":"10.32471/exp-oncology.2312-8852.vol-44-no-3.18434","DOIUrl":null,"url":null,"abstract":"Background: The anticancer effects of phytohormones of cytokinin nature are similar to those of medicinal mushrooms, which are able to synthesize cytokinins in large amounts.Aim: To determine the antiproliferative effect of crude extracts and cytokinin fractions from the mycelial biomass of seven fungi species on colon cancer cells in vitro.Materials and methods: Cytokinin content in mycelial biomass of Ganoderma lucidum, Lentinula edodes, Trametes versicolor, Pleurotus ostreatus, Morchella esculenta, Hericium coralloides, and Fomitopsis officinalis was determined by high performance liquid chromatography mass spectrometry. The antiproliferative effect of the mushroom extracts on the human colon adenocarcinoma Colo 205 cells was assessed by MTT-test.Results: The content of cytokinins (trans-zeatin, zeatin riboside, isopentenyladenosine, isopentenyladenine and zeatin-O-glucoside) was determined in the mycelial biomass of the medicinal macromycetes. Zeatin-type hormones prevailed in all species, though trans-zeatin was the most abundant in H. coralloides and M. esculenta. In P. ostreatus, only zeatin-O-glucoside was detected. The lowest IC50 was found for both the cytokinin fraction (0.21 μg/ml) and the crude extract (0.17 μg/ml) from mycelial biomass of H. coralloides. F. officinalis also demonstrated high antiproliferative effect against Colo 205 cells: IC50 was 0.9 μg/ml for the crude extract and almost twice lower for the cytokinin fraction. In the studied concentration range (0.016-2 μg/ml), the crude extracts from G. lucidum and M. esculenta and the cytokinin fraction from L. edodes did not reach IC50 values.Conclusions: The present study showed that crude extracts and/or cytokinin fractions of several medicinal Basidiomycetes species are capable to inhibit proliferation of colon cancer cells in vitro. Crude extract cytotoxicity of H. coralloides, P. ostreatus and T. versicolor was higher than that of cytokinin fraction while antiproliferative effect of cytokinin fraction from F. officinalis was higher than that in its crude extract.","PeriodicalId":12287,"journal":{"name":"Experimental oncology","volume":"44 3","pages":"213-216"},"PeriodicalIF":0.0000,"publicationDate":"2022-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Experimental oncology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.32471/exp-oncology.2312-8852.vol-44-no-3.18434","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"Medicine","Score":null,"Total":0}

引用次数: 1

Abstract

Background: The anticancer effects of phytohormones of cytokinin nature are similar to those of medicinal mushrooms, which are able to synthesize cytokinins in large amounts.

Aim: To determine the antiproliferative effect of crude extracts and cytokinin fractions from the mycelial biomass of seven fungi species on colon cancer cells in vitro.

Materials and methods: Cytokinin content in mycelial biomass of Ganoderma lucidum, Lentinula edodes, Trametes versicolor, Pleurotus ostreatus, Morchella esculenta, Hericium coralloides, and Fomitopsis officinalis was determined by high performance liquid chromatography mass spectrometry. The antiproliferative effect of the mushroom extracts on the human colon adenocarcinoma Colo 205 cells was assessed by MTT-test.

Results: The content of cytokinins (trans-zeatin, zeatin riboside, isopentenyladenosine, isopentenyladenine and zeatin-O-glucoside) was determined in the mycelial biomass of the medicinal macromycetes. Zeatin-type hormones prevailed in all species, though trans-zeatin was the most abundant in H. coralloides and M. esculenta. In P. ostreatus, only zeatin-O-glucoside was detected. The lowest IC50 was found for both the cytokinin fraction (0.21 μg/ml) and the crude extract (0.17 μg/ml) from mycelial biomass of H. coralloides. F. officinalis also demonstrated high antiproliferative effect against Colo 205 cells: IC50 was 0.9 μg/ml for the crude extract and almost twice lower for the cytokinin fraction. In the studied concentration range (0.016-2 μg/ml), the crude extracts from G. lucidum and M. esculenta and the cytokinin fraction from L. edodes did not reach IC50 values.

Conclusions: The present study showed that crude extracts and/or cytokinin fractions of several medicinal Basidiomycetes species are capable to inhibit proliferation of colon cancer cells in vitro. Crude extract cytotoxicity of H. coralloides, P. ostreatus and T. versicolor was higher than that of cytokinin fraction while antiproliferative effect of cytokinin fraction from F. officinalis was higher than that in its crude extract.

查看原文本刊更多论文

几种药用担子菌菌丝体提取物对结肠癌细胞COLO 205的抑增殖作用。

背景:天然细胞分裂素类植物激素的抗癌作用与药用蘑菇相似，均能大量合成细胞分裂素。目的:研究7种真菌菌丝体粗提物和细胞分裂素组分对结肠癌细胞的体外抗增殖作用。材料与方法:采用高效液相色谱-质谱法测定了灵芝、香菇、花斑菌、平菇、羊肚菌、黑孢菌和officinalis菌丝生物量中细胞分裂素的含量。采用mtt法评价了香菇提取物对人结肠腺癌Colo 205细胞的抗增殖作用。结果:测定了药用大型菌菌丝生物量中细胞分裂素(反式玉米素、玉米素核苷、异戊烯腺苷、异戊烯腺苷、玉米素o糖苷)的含量。玉米素型激素在所有物种中均占主导地位，但反式玉米素在玉米叶蚜和玉米叶蚜中含量最高。其中，玉米苷- o -葡萄糖苷仅被检测到。细胞分裂素部分(0.21 μg/ml)和粗提物(0.17 μg/ml)的IC50最低。对Colo 205细胞也表现出较高的抗增殖作用:粗提物的IC50为0.9 μg/ml，细胞分裂素部分的IC50几乎低2倍。在研究浓度范围(0.016 ~ 2 μg/ml)内，灵芝、牛蒡粗提物和香薷细胞分裂素部位均未达到IC50值。结论:本研究表明，几种药用担子菌的粗提物和/或细胞分裂素组分在体外具有抑制结肠癌细胞增殖的作用。粗提物的细胞毒性高于粗提物的细胞分裂素部位，粗提物的细胞分裂素部位的细胞增殖作用高于粗提物的细胞分裂素部位。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Experimental oncology Medicine-Oncology

CiteScore

1.40

自引率

0.00%

发文量

期刊介绍： The Experimental Oncology is an English-language journal that publishes review articles, original contributions, short communications, case reports and technical advances presenting new data in the field of experimental and fundamental oncology. Manuscripts should be written in English, contain original work, which has not been published or submitted for publication elsewhere. It also implies the transfer of the Copyright from the author to “Experimental Oncology”. No part of journal publications may be reproduced, stored in a retrieval system or transmitted in any form or by any means without the prior permission of the publisher.