Kinetics of Granuloma, IFN-γ and IP-10 in a Wistar Rat Model Infected with Mycobacterium Tuberculosis.

Abstract

Background: Improved access and treatment are critical to controlling the problem. Molecular diagnostic tests, although available, are not feasible in developing countries. Where available, these tests require state-of-the-art laboratories and are not cheap. One alternative to molecular diagnostics is the use of acute phase protein values. This protein is a protein whose levels will increase or decrease in plasma in response to injury or inflammation.

Objective: This study aimed to analyze the kinetics of granulomas and the role of IFN-γ and IP-10 in the pathology of tuberculosis in a rat model.

Methods: Sixty Wistar rats were divided into four groups, namely the control group (without MTB induction) and the MTB-induced group (observations at week-3, week-6, and week-12 post infection). Induce tuberculosis with bacterial strain H37Rv ATCC 27294.

Results: The number and size of the granuloma increased to a peak at week 6 and was consistent for weeks 6 and 12 post-infection. The kinetics of granulomas were consistent with IFN-γ and IP-10 levels.

Conclusion: It was concluded that the model of tuberculosis infection by the H37Rv ATCC 27294 strain in Wistar rat found granuloma characteristics and IFN-γ and IP-10 patterns in similar kinetics, so that there was involvement of these molecules in TB pathology. Thus, tuberculosis infection of the H37Rv ATCC 27294 strain in rats can serve as a model for rapid tuberculosis study and display a complete pathological phase.