Polymorphism Pro₁₂ --Ala of the peroxisome proliferator-activated receptors gamma gene effect on lipid peroxidation and antioxidant defense in patients with type 2 diabetes.

Abstract

In this research, we discover effect ofpolymorphism rs1801282 of the PPARG gene on the formation oxidative stress in patients with type 2 diabetes, depending on the duration of the disease: till 5 years, 5-10 years and more than 10 years. Activity of lipidperoxidation was assess in terms of diene conjugates (DC) and malonic dealdehid (MDA) and antioxidant system status - the activity of superoxide dismutase (SOD), catalase and the level of a-tocopherol (a-TF). Molecular genetic studies conducted by the method ofpolymerase chain reaction (PCR) in real time. During disease 5-10 years, found increase levels of DC and MDA in patients with polymorphism Pro₁₂--Ala for 34.9 and 34.7%, in compared with ProPro₁₂ --Pro (P=O. 01). Availability ProPro₁₂ --Pro stipulated reduction of catalase activity during disease 5-10 years at 75% (P=O. 001), andfor those, who are illfor more than 10 years at 2,04 times (P=O.01), which is not different from the reference level (F=1. 19; P=O. 600), but in the case ProPro₁₂ --Ala, this figure was in 2 times higher The main conclu- sion is that the type ProPro₁₂ --+Ala ofpolymorphism rs1801282 of the PPARG gene causes the development of oxidative stress in patients with type 2 diabetes with 5-10 years durations, and Pro Pro₁₂ --Pro - deficiency the enzyme catalase level of antioxidant system in patients with durations of disease more than 5 years.