Understanding Role of DNA Repair and Cytochrome p-450 Gene Polymorphisms in Cervical Cancer Patient Treated With Concomitant Chemoradiation.

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS
ACS Applied Bio Materials Pub Date : 2022-02-24 eCollection Date: 2022-01-01 DOI:10.3389/bjbs.2021.10120
Mohammad Abbas, Vandana Singh Kushwaha, Kirti Srivastava, Monisha Banerjee
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引用次数: 3

Abstract

Background: Evidences suggest that single nucleotide polymorphisms (SNPs) can be considered as potential biomarkers for disease progression and therapeutic response in cervical cancer. The present study investigated the association of CYP1A1 T>C (rs4646903), CYP1A1 A>G (rs1048943), CYP2E1 T>A (rs6413432), RAD51 G>C (rs1801320), XRCC1 G>A (rs25487), XRCC2 G>A (rs3218536) and XRCC3 C>T (rs861539) polymorphisms with treatment outcome of cisplatin based chemoradiation (CRT). Methods: Total 227 cervical cancer cases, treated with the same chemoradiotherapy regimen were selected for the study. Genotyping analysis was performed by PCR-restriction fragment length polymorphisms (PCR-RFLP). Treatment response was evaluated by Response Evaluation Criteria in Solid Tumors (RECIST). Association of all clinical data (responses, recurrence and survival of patients) and single nucleotide polymorphisms (SNPs) was analysed by using SPSS (version 21.0). Results: Patients with TA/AA genotype of CYP2E1 T>A polymorphism showed significantly poor response while those with GC/CC genotype of RAD51 G>C showed better response (p = 0.008, p = 0.014 respectively). Death was significantly higher in patients with GG genotypes of RAD51 G>C and XRCC1 G>A (p = 0.006, p = 0.002 respectively). Women with GC+CC genotype of RAD51 G>C and AG+GG of XRCC1 showed better survival and also reduced risk of death (HR = 0.489, p = 0.008; HR = 0.484, p = 0.003 respectively). Conclusion: Results suggested that CYP2E1 T>A (rs6413432), RAD51 G>C (rs1801320), and XRCC1 G>A (rs25487) polymorphisms may be used as predictive markers for clinical outcomes in cervical cancer patients undergoing cisplatin based concomitant chemoradiotherapy.

Abstract Image

DNA修复和细胞色素p-450基因多态性在宫颈癌放化疗患者中的作用
背景:有证据表明,单核苷酸多态性(snp)可以被认为是宫颈癌疾病进展和治疗反应的潜在生物标志物。本研究探讨了CYP1A1 T>C (rs4646903)、CYP1A1 A>G (rs1048943)、CYP2E1 T>A (rs6413432)、RAD51 G>C (rs1801320)、XRCC1 G>A (rs25487)、XRCC2 G>A (rs3218536)和XRCC3 C>T (rs861539)多态性与顺铂化放化疗(CRT)治疗结果的关系。方法:选择采用相同放化疗方案的宫颈癌患者227例作为研究对象。采用pcr -限制性片段长度多态性(PCR-RFLP)进行基因分型分析。采用实体瘤反应评价标准(RECIST)评价治疗反应。使用SPSS (version 21.0)分析所有临床数据(疗效、复发和患者生存)与单核苷酸多态性(snp)的关系。结果:CYP2E1 T>A多态性的TA/AA基因型患者疗效较差,而RAD51 G>C基因型的GC/CC患者疗效较好(p = 0.008, p = 0.014)。RAD51 G>C、XRCC1 G>A的GG基因型患者死亡率显著高于其他基因型(p = 0.006、p = 0.002)。RAD51 G>C基因型为GC+CC、XRCC1基因型为AG+GG的女性生存率更高,死亡风险降低(HR = 0.489, p = 0.008;HR = 0.484, p = 0.003)。结论:CYP2E1 T>A (rs6413432)、RAD51 G>C (rs1801320)、XRCC1 G>A (rs25487)多态性可作为顺铂联合放化疗宫颈癌患者临床预后的预测指标。
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来源期刊
ACS Applied Bio Materials
ACS Applied Bio Materials Chemistry-Chemistry (all)
CiteScore
9.40
自引率
2.10%
发文量
464
期刊介绍: ACS Applied Bio Materials is an interdisciplinary journal publishing original research covering all aspects of biomaterials and biointerfaces including and beyond the traditional biosensing, biomedical and therapeutic applications. The journal is devoted to reports of new and original experimental and theoretical research of an applied nature that integrates knowledge in the areas of materials, engineering, physics, bioscience, and chemistry into important bio applications. The journal is specifically interested in work that addresses the relationship between structure and function and assesses the stability and degradation of materials under relevant environmental and biological conditions.
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