Identification of Cleaved Haptoglobin in the Serum of Bee Venom-Hypersensitive Patients.

IF 0.9 Q4 INTEGRATIVE & COMPLEMENTARY MEDICINE
Jae-Min Ryu, Han-Heom Na, Jin-Sung Park, Byung-Soo Ahn, Keun-Cheol Kim
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引用次数: 1

Abstract

Background: Bee venom has been used as a therapeutic compound for various human diseases in oriental medicine; however, it can induce anaphylaxis in hypersensitive patients during treatment. Anaphylaxis is an acute allergic reaction that occurs after allergen exposure. IgE is released from immune-related cells such as mast cells and basophils during anaphylaxis. Various inflammatory mediators are also released into the bloodstream during the acute response.

Objectives: We aimed to identify specific proteins from bee venom-hypersensitive patients.

Methods: We analyzed the blood serum of control and bee venom-hypersensitive patients using two-dimensional (2D) electrophoresis.

Results: An interesting protein spot with a molecular size of 10 kDa was identified at an isoelectric point (p.I.) of 5.5. Spots detected both before and after sweet bee venom therapy were not proteins induced by sweet bee venom. The 10 kDa protein was identified as the cleaved form of haptoglobin through liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis. Statistical analysis indicated that the presence of the spot was highly significant in the bee venom-hypersensitive group.

Conclusion: The findings suggest that cleaved haptoglobin may be a significant diagnostic protein for anaphylaxis. In addition, a high incidence of bee venom hypersensitivity may be associated with the haptoglobin genotype.

蜜蜂毒液过敏患者血清中裂裂型触珠蛋白的鉴定。
背景:在东方医学中,蜂毒已被用作治疗各种人类疾病的药物;然而,在治疗过程中,它可以引起过敏反应的过敏患者。过敏反应是在接触过敏原后发生的急性过敏反应。在过敏反应期间,IgE从免疫相关细胞如肥大细胞和嗜碱性细胞释放出来。在急性反应期间,各种炎症介质也被释放到血液中。目的:我们旨在鉴定蜜蜂毒液过敏患者的特异性蛋白质。方法:采用二维电泳法对对照组和蜂毒过敏患者的血清进行分析。结果:在等电点(pi)为5.5处发现了一个分子量为10 kDa的有趣蛋白点。甜蜂毒治疗前后检测到的斑点均不是甜蜂毒诱导的蛋白。通过液相色谱-串联质谱(LC-MS/MS)分析,鉴定了10 kDa蛋白为裂裂形式的触珠蛋白。统计分析表明,该斑点的存在是高度显著的蜜蜂毒液过敏组。结论:裂化接触珠蛋白可能是一种重要的过敏反应诊断蛋白。此外,蜂毒过敏的高发可能与触珠蛋白基因型有关。
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来源期刊
Journal of Acupuncture and Meridian Studies
Journal of Acupuncture and Meridian Studies INTEGRATIVE & COMPLEMENTARY MEDICINE-
CiteScore
1.50
自引率
10.00%
发文量
35
审稿时长
12 weeks
期刊介绍: The Journal of Acupuncture and Meridian Studies is a quarterly, peer-reviewed journal featuring high-quality studies related to basic and clinical acupuncture and meridian research. It also includes new paradigm of integrative research, covering East–West and traditional–modern medicine. Manuscripts should fall into one of the categories: topical review, original research paper, brief ... click here for full Aims & Scope The Journal of Acupuncture and Meridian Studies is a quarterly, peer-reviewed journal featuring high-quality studies related to basic and clinical acupuncture and meridian research. It also includes new paradigm of integrative research, covering East–West and traditional–modern medicine.
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