Characterization of IL-10-producing regulatory B cells in thymoma.

IF 3.3 4区医学 Q3 IMMUNOLOGY

Autoimmunity Pub Date : 2022-09-01 Epub Date: 2022-06-29 DOI:10.1080/08916934.2022.2093862

Fangnan Lin, Hailong You, Xiwen Cao, Tingting Li, Xiaodong Hong, Jinli Yang, Peng Huo, Jialin Li, Wei Liu, Yanfang Jiang

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引用次数: 1

Abstract

Background: Regulatory B cells (Bregs) are a subset of B cells that secrete interleukin 10 (IL-10) and play a vital role in suppressing the immune response. The aim of this study was to evaluate the proportion of Bregs in patients with thymoma.

Methods: The proportions of subgroups of Bregs in 23 patients with thymoma and 15 healthy controls were detected by flow cytometry. The serum IL-2, IL-4, IL-6, IL-10, IL-17A, IFN-γ, and TNF-α levels of the subjects were measured using a cytometric bead array (CBA).

Results: The proportions of circulating IL-10⁺ B cells, IL-10⁺CD24^hiCD38^hi Bregs, and IL-10⁺CD24^hiCD27⁺ Bregs and the serum IL-10 level were significantly higher in patients with thymoma than in the control group and were negatively correlated with the Karnofsky Performance Scale (KPS) score. The serum levels of cytokines IL-2, IL-6, IFN-γ, and TNF-α were higher and serum IL-17A level was lower in patients with thymoma. Patients with advanced-stage thymoma exhibited significantly higher proportions of IL-10-producing Bregs and a higher serum IL-10 level. After tumour resection, the frequency of circulating IL-10⁺CD24^hiCD38^hi Bregs and the serum IL-10 level were significantly decreased in patients with thymoma. The serum IL-10 levels exhibited the best accuracy in assessing the risk of thymoma occurrence in this study.

Conclusions: The expression of IL-10 produced by Bregs is increased in patients with thymoma, particularly those with advanced-stage disease, which may suggest that Bregs are involved in the pathogenesis and progression of thymoma.

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胸腺瘤中产生il -10的调节性B细胞的表征。

背景:调节性B细胞(Regulatory B cells, Bregs)是分泌白细胞介素10 (interleukin 10, IL-10)的B细胞子集，在抑制免疫应答中发挥重要作用。本研究的目的是评估胸腺瘤患者中Bregs的比例。方法:采用流式细胞术检测23例胸腺瘤患者和15例健康对照者血清Bregs亚群比例。采用细胞头阵列(CBA)检测血清IL-2、IL-4、IL-6、IL-10、IL-17A、IFN-γ和TNF-α水平。结果:胸腺瘤患者外周血IL-10+ B细胞比例、IL-10+CD24hiCD38hi Bregs、IL-10+CD24hiCD27+ Bregs及血清IL-10水平均显著高于对照组，且与Karnofsky绩效量表(KPS)评分呈负相关。胸腺瘤患者血清中细胞因子IL-2、IL-6、IFN-γ、TNF-α水平较高，IL-17A水平较低。晚期胸腺瘤患者表现出更高比例的产生IL-10的Bregs和更高的血清IL-10水平。肿瘤切除后，胸腺瘤患者循环IL-10+CD24hiCD38hi Bregs频率及血清IL-10水平均显著降低。在本研究中，血清IL-10水平在评估胸腺瘤发生风险方面表现出最好的准确性。结论:Bregs产生的IL-10在胸腺瘤患者，尤其是晚期胸腺瘤患者中表达升高，提示Bregs可能参与了胸腺瘤的发病和进展。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Autoimmunity 医学-免疫学

CiteScore

5.70

自引率

8.60%

发文量

审稿时长

6-12 weeks

期刊介绍： Autoimmunity is an international, peer reviewed journal that publishes articles on cell and molecular immunology, immunogenetics, molecular biology and autoimmunity. Current understanding of immunity and autoimmunity is being furthered by the progress in new molecular sciences that has recently been little short of spectacular. In addition to the basic elements and mechanisms of the immune system, Autoimmunity is interested in the cellular and molecular processes associated with systemic lupus erythematosus, rheumatoid arthritis, Sjogren syndrome, type I diabetes, multiple sclerosis and other systemic and organ-specific autoimmune disorders. The journal reflects the immunology areas where scientific progress is most rapid. It is a valuable tool to basic and translational researchers in cell biology, genetics and molecular biology of immunity and autoimmunity.