Serum exosomal microRNA-370-3p and microRNA-196a-5p are potential biomarkers for the diagnosis and prognosis of hepatocellular carcinoma.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2022-01-01 Epub Date: 2022-06-28 DOI:10.5603/FHC.a2022.0019

Yaning Wei, Qian Zhang, Lin An, Guotao Fang, Dan Hong, Ting Jiao, Hua Yang, Zhiyu Wang

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引用次数: 5

Abstract

Introduction: Evidence has shown that some microRNAs (miRNAs) play a role in tumorigenesis of hepatocellular carcinoma (HCC). Herein, we aimed to evaluate the diagnostic and prognostic values of serum exosomal miR-370-3p and miR-196a-5p in patients with HCC.

Material and methods: Serum exosomes in 90 HCC patients were extracted and identified. Serum exosomal miR-370-3p and miR-196a-5p expression in HCC patients were detected. The diagnostic value of miR-370-3p and miR-196a- 5p, relationship between miR-370-3p and miR-196a-5p expression and clinicopathological features and prognosis of patients with HCC were analyzed. Relationship between miR-370-3p and miR-196a-5p expression and liver function indices such as alanine aminotransferase (ALT), aspartate aminotransferase (AST), and total bilirubin (TBIL) in HCC patients were analyzed. The effects of miR-370-3p and miR-196a-5p on Huh-7 HCC cells' proliferation, invasion and migration were determined.

Results: Lower expression of miR-370-3p and higher expression of miR-196a-5p were found in serum exosomes of HCC patients. Serum exosomal miR-370-3p and miR-196a-5p were associated with tumor size, tumor grade and TNM stage as well as prognosis and liver function indices of HCC patients. Overexpressed miR-370-3p or silenced miR-196a-5p suppressed proliferation, invasion and migration of Huh-7 HCC cells.

Conclusions: We suggest that miR-370-3p/miR-196a-5p in serum exosomes of HCC patients could be potential biomarkers for the diagnosis and prognosis of HCC.

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血清外泌体microRNA-370-3p和microRNA-196a-5p是肝细胞癌诊断和预后的潜在生物标志物。

有证据表明，一些microRNAs (miRNAs)在肝细胞癌(HCC)的肿瘤发生中起作用。在此，我们旨在评估血清外泌体miR-370-3p和miR-196a-5p在HCC患者中的诊断和预后价值。材料与方法:对90例HCC患者的血清外泌体进行了提取鉴定。检测HCC患者血清外泌体miR-370-3p和miR-196a-5p的表达。分析miR-370-3p和miR-196a-5p的诊断价值，以及miR-370-3p和miR-196a-5p表达与HCC患者临床病理特征和预后的关系。分析miR-370-3p、miR-196a-5p表达与HCC患者肝功能指标如谷丙转氨酶(ALT)、天冬氨酸转氨酶(AST)、总胆红素(TBIL)的关系。检测miR-370-3p和miR-196a-5p对Huh-7 HCC细胞增殖、侵袭和迁移的影响。结果:HCC患者血清外泌体中miR-370-3p表达较低，miR-196a-5p表达较高。血清外泌体miR-370-3p和miR-196a-5p与HCC患者肿瘤大小、肿瘤分级、TNM分期以及预后和肝功能指标相关。过表达的miR-370-3p或沉默的miR-196a-5p抑制Huh-7 HCC细胞的增殖、侵袭和迁移。结论:我们认为HCC患者血清外泌体中的miR-370-3p/miR-196a-5p可能是HCC诊断和预后的潜在生物标志物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.