MicroRNA-877-5p Inhibits Cell Progression by Targeting FOXM1 in Lung Cancer.

IF 2.1 4区医学 Q3 RESPIRATORY SYSTEM

Canadian respiratory journal Pub Date : 2022-06-15 eCollection Date: 2022-01-01 DOI:10.1155/2022/4256172

Zhiguang Liu, Xinlian Wang, Liqiang Cao, Xiaowei Yin, Qian Zhang, Lan Wang

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引用次数: 2

Abstract

Background: Many researches revealed that microRNAs (miRNAs) function as potential oncogene or tumor suppressor gene. As an antioncogene, miR-877-5p was reduced in many tumors.

Objective: This research aimed to explore the biological role and mechanisms of miR-877-5p, which may help patients with non-small-cell lung cancer (NSCLC) find effective therapeutic targets.

Methods: The downstream targets of miR-877-5p were predicted by Bioinformatics software. RT-qPCR and western blot were employed to analyze the gene levels. The impacts of miR-877-5p and FOXM1 were assessed by cell function experiments.

Results: The miR-877-5p was reduced in NSCLC. In addition to this, it also inhibited cell progression of NSCLC cells in vitro. Moreover, the upregulation of FOXM1 expression restored the inhibitory effect of enhancement of miR-877-5p.

Conclusions: Taken together, miR-877-5p inhibited cell progression by directly targeting FOXM1, which may provide potential biomarkers for targeted therapy of NSCLC.

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MicroRNA-877-5p靶向肺癌FOXM1抑制细胞进展

背景:许多研究表明，microRNAs (miRNAs)具有潜在的致癌基因或肿瘤抑制基因的功能。作为一个反基因，miR-877-5p在许多肿瘤中表达降低。目的:本研究旨在探讨miR-877-5p的生物学作用及机制，以期帮助非小细胞肺癌(NSCLC)患者找到有效的治疗靶点。方法:利用生物信息学软件预测miR-877-5p的下游靶点。采用RT-qPCR和western blot分析基因表达水平。通过细胞功能实验评估miR-877-5p和FOXM1的影响。结果:miR-877-5p在NSCLC中降低。除此之外，它还能在体外抑制NSCLC细胞的细胞进展。此外，FOXM1表达上调恢复了miR-877-5p增强的抑制作用。结论:综上所述，miR-877-5p通过直接靶向FOXM1抑制细胞进展，这可能为NSCLC的靶向治疗提供潜在的生物标志物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Canadian respiratory journal 医学-呼吸系统

CiteScore

4.20

自引率

0.00%

发文量

审稿时长

6-12 weeks

期刊介绍： Canadian Respiratory Journal is a peer-reviewed, Open Access journal that aims to provide a multidisciplinary forum for research in all areas of respiratory medicine. The journal publishes original research articles, review articles, and clinical studies related to asthma, allergy, COPD, non-invasive ventilation, therapeutic intervention, lung cancer, airway and lung infections, as well as any other respiratory diseases.