Genetic Characterization and Antimicrobial-Resistant Profiles of Staphylococcus aureus Isolated from Different Food Sources.

IF 0.9 4区农林科学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biocontrol science Pub Date : 2022-01-01 DOI:10.4265/bio.27.87

Reem Ghabbour, Amal Awad, Gamal Younis

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引用次数: 1

Abstract

This study determined the prevalence of Staphylococcus aureus in food of animal origin, investigated its antimicrobial susceptibility profiles and antimicrobial-resistant genes encoding resistance to methicillin (mecA), penicillin (blaZ), and vancomycin (vanA). Two hundred and sixty food samples, including raw retail milk, meat, and meat products, were obtained from local retail shops in Mansoura city, Egypt. The overall prevalence of S. aureus in the total examined food samples was 32.69% (85/260). Methicillin-resistant S. aureus (MRSA) was identified in 11.15% (29/260) of the tested food samples. S. aureus indicated a high resistance to nalidixic acid, penicillin, ampicillin, cefuroxime, trimethoprim/sulfamethoxazole, and azithromycin. The multiple antibiotic resistance (MAR) rate was 89.4% of the total S. aureus isolates, and MARindex ranges from 0.05-0.64. Genotypically, mecA and blaZ genes were identified in a percentage of 34.11% and 82.35%, respectively, while no isolates harbored the vanA gene. The presence of MAR S. aureus particularly, MRSA in food samples, is of great concern and represents a possible threat to the community. Therefore, the study's findings highlight the importance of establishing vigilant food safety practices for food handlers to inhibit the transmission of S. aureus through the food chain to reduce public health risks.

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从不同食物来源分离的金黄色葡萄球菌的遗传特征和耐药谱。

本研究确定了金黄色葡萄球菌在动物源性食品中的流行情况，研究了其对甲氧西林(mecA)、青霉素(blaZ)和万古霉素(vanA)的耐药基因。从埃及曼苏拉市当地零售商店取得260份食品样本，包括零售生奶、肉类和肉制品。金黄色葡萄球菌在全部检测食品样品中的总体检出率为32.69%(85/260)。耐甲氧西林金黄色葡萄球菌(MRSA)检出率为11.15%(29/260)。金黄色葡萄球菌对萘啶酸、青霉素、氨苄西林、头孢呋辛、甲氧苄啶/磺胺甲恶唑和阿奇霉素具有高度耐药性。多重耐药率为89.4%，多重耐药指数为0.05 ~ 0.64。从基因特征上看，mea和blaZ基因分别占34.11%和82.35%，而vanA基因未检出。金黄色葡萄球菌的存在，特别是在食品样品中的MRSA，是非常令人担忧的，并代表了对社会的可能威胁。因此，该研究的发现强调了为食品处理人员建立警惕的食品安全措施以抑制金黄色葡萄球菌通过食物链传播以减少公共卫生风险的重要性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biocontrol science BIOTECHNOLOGY & APPLIED MICROBIOLOGY-

CiteScore

2.60

自引率

8.30%

发文量

审稿时长

>12 weeks

期刊介绍： The Biocontrol Science provides a medium for the publication of original articles, concise notes, and review articles on all aspects of science and technology of biocontrol.