Knockdown circTRIM28 enhances tamoxifen sensitivity via the miR-409-3p/HMGA2 axis in breast cancer.

Reproductive biology and endocrinology : RB&E Pub Date : 2022-09-30 DOI:10.1186/s12958-022-01011-3

Shiyong Yang, Changwu Zou, Yuxin Li, Xianguo Yang, Wei Liu, Guannan Zhang, Nina Lu

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引用次数: 4

Abstract

Background: Tamoxifen (TAM) is a frequently-used treatment for breast cancer (BC). But the TAM resistance seriously affects the patient therapeutic effect. Previous research indicated that circular RNAs (circRNAs) might participate in the regulatory processes of BC. Here, we discovered the parts of circular RNA tripartite motif-containing 28 (circTRIM28) in BC.

Methods: CircTRIM28, microRNA-409-3p (miR-409-3p), and high mobility group AT-hook 2 (HMGA2) levels were perceived by qRT-PCR and western blot. Moreover, the biological functions of the cells were examined. Furthermore, dual-luciferase report was employed to reconnoiter the targeted relationship between miR-409-3p and circTRIM28 or HMGA2.

Results: CircTRIM28 and HMGA2 were augmented, and the miR-409-3p was repressed in BC. Silencing circTRIM28 enhanced tamoxifen sensitivity and cell apoptosis, whereas hampered cell development in BC cells. In mechanism, circTRIM28 could sponge miR-409-3p to increase HMGA2. In addition, silencing circTRIM28 impeded tumor growth.

Conclusion: CircTRIM28 facilitated the BC via miR-409-3p/HMGA2.

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敲低circTRIM28通过miR-409-3p/HMGA2轴增强乳腺癌中他莫昔芬的敏感性。

背景:他莫昔芬(TAM)是乳腺癌(BC)的常用治疗药物。但TAM耐药严重影响患者的治疗效果。先前的研究表明，环状rna (circRNAs)可能参与BC的调控过程。我们在BC中发现了环状RNA三基序28 (circTRIM28)的部分。方法:采用qRT-PCR和western blot检测CircTRIM28、microRNA-409-3p (miR-409-3p)、高迁移率组AT-hook 2 (HMGA2)水平。并对细胞的生物学功能进行了检测。此外，采用双荧光素酶报告来探测miR-409-3p与circTRIM28或HMGA2之间的靶向关系。结果:BC中CircTRIM28和HMGA2表达增强，miR-409-3p表达抑制。沉默circTRIM28增强了他莫昔芬敏感性和细胞凋亡，而抑制了BC细胞的细胞发育。在机制上，circTRIM28可以海绵miR-409-3p增加HMGA2。此外，沉默cirtrim28抑制肿瘤生长。结论:CircTRIM28通过miR-409-3p/HMGA2促进BC。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Reproductive biology and endocrinology : RB&E

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