Effects of a graphene oxide-alginate sheet scaffold on rotator cuff tendon healing in a rat model.

IF 1.6
Jong Pil Yoon, Dong Hyun Kim, Seung Gi Min, Hun-Min Kim, Jin-Hyun Choi, Hyun Joo Lee, Kyeong Hyeon Park, Seong Soo Kim, Seok Won Chung, Sung Hyuk Yoon
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引用次数: 1

Abstract

Background: Natural polymer scaffolds used to promote rotator cuff healing have limitations in terms of their mechanical and biochemical properties. This animal study aimed to investigate the effects of combined graphene oxide (GO) and alginate scaffold and the toxicity of GO on rotator cuff healing in a rat model.

Methods: First, the mechanical properties of a GO/alginate scaffold and a pure alginate scaffold were compared. The in vitro cytotoxicity of and proliferation of human tenocytes with the GO/alginate scaffold were evaluated by CCK-8 assay. For the in vivo experiment, 20 male rats were randomly divided into two groups (n = 10 each), and supraspinatus repair was performed: group 1 underwent supraspinatus repair alone, and group 2 underwent supraspinatus repair with the GO/alginate scaffold. Biomechanical and histological analyses were performed to evaluate the quality of tendon-to-bone healing 8 weeks after rotator cuff repair.

Results: The GO/alginate scaffold exhibited an increased maximum load (p = .001) and tensile strength (p = .001). In the cytotoxicity test, the cell survival rate with the GO/alginate scaffold was 102.08%. The proliferation rate of human tenocytes was no significant difference between the GO/alginate and alginate groups for 1, 3, 5, and 7 days. Biomechanically, group 2 exhibited a significantly greater ultimate failure load (p < .001), ultimate stress (p < .001), and stiffness (p < .001) than group 1. The histological analysis revealed that the tendon-to-bone interface in group 2 showed more collagen fibers bridging, tendon-to-bone integration, longitudinally oriented collagen fibers, and fibrocartilage formation than in group 1.

Conclusion: A small amount of GO added to alginate improved the mechanical properties of the scaffold without evidence of cytotoxicity. At 8 weeks after rotator cuff repair, the GO/alginate scaffold improved tendon-to-bone healing without causing any signs of toxicity in a rat model.

氧化石墨烯-海藻酸盐薄片支架对大鼠模型中肩袖肌腱愈合的影响。
背景:用于促进肩袖愈合的天然聚合物支架在其机械和生化特性方面存在局限性。本动物研究旨在探讨氧化石墨烯(GO)和海藻酸盐复合支架对大鼠模型中肩袖愈合的影响以及GO的毒性。方法:首先,比较氧化石墨烯/海藻酸盐支架和纯海藻酸盐支架的力学性能。CCK-8法检测氧化石墨烯/海藻酸盐支架对人细胞的体外细胞毒性和增殖能力。体内实验将20只雄性大鼠随机分为两组(每组10只),分别进行冈上肌修复:1组单独修复冈上肌,2组采用氧化石墨烯/海藻酸盐支架修复冈上肌。进行生物力学和组织学分析,评估肩袖修复后8周肌腱-骨愈合的质量。结果:氧化石墨烯/海藻酸盐支架显示出增加的最大载荷(p = .001)和抗拉强度(p = .001)。在细胞毒性试验中,氧化石墨烯/海藻酸盐支架的细胞存活率为102.08%。1、3、5、7天,氧化石墨烯/海藻酸盐组和海藻酸盐组的人细胞增殖率无显著差异。生物力学方面,2组的极限破坏载荷(p < 0.001)、极限应力(p < 0.001)和刚度(p < 0.001)显著高于1组。组织学分析显示,与1组相比,2组肌腱-骨界面显示更多的胶原纤维桥接,肌腱-骨融合,纵向定向的胶原纤维和纤维软骨形成。结论:在海藻酸盐中添加少量氧化石墨烯可以改善支架的力学性能,但没有细胞毒性的证据。在肌腱套修复后8周,在大鼠模型中,氧化石墨烯/海藻酸盐支架改善了肌腱到骨的愈合,没有引起任何毒性迹象。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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