miR-181a-5p is a potential candidate epigenetic biomarker in multiple sclerosis.

IF 2.3 3区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Genome Pub Date : 2022-11-01 Epub Date: 2022-09-14 DOI:10.1139/gen-2022-0040
Tuba Gökdoğan Edgünlü, Şenay Görücü Yılmaz, Ufuk Emre, Bahar Taşdelen, Oktay Kuru, Gülnihal Kutlu, Mehmet Emin Erdal
{"title":"miR-181a-5p is a potential candidate epigenetic biomarker in multiple sclerosis.","authors":"Tuba Gökdoğan Edgünlü,&nbsp;Şenay Görücü Yılmaz,&nbsp;Ufuk Emre,&nbsp;Bahar Taşdelen,&nbsp;Oktay Kuru,&nbsp;Gülnihal Kutlu,&nbsp;Mehmet Emin Erdal","doi":"10.1139/gen-2022-0040","DOIUrl":null,"url":null,"abstract":"<p><p>Multiple sclerosis (MS) is a chronic inflammatory disease of the central nervous system (CNS) characterized by demyelination and axonal degeneration. Abnormal expression of microRNAs (miRNAs) plays an important role in MS pathology. In this cohort study, differential expression of the four miRNAs (<i>hsa-miR-155-5p</i>, <i>hsa-miR-9-5p</i>, <i>hsa-miR-181a-5p</i>, and <i>hsa-miR-125b-5p)</i> was investigated in 69 individuals, including 39 MS patients (relapsing-remitting MS (RRMS), <i>n</i> = 27; secondary progressive MS (SPMS), <i>n</i> = 12) and 30 healthy controls. In silico analyses revealed possible genes and pathways specific to miRNAs. Peripheral blood miRNA expressions were detected by quantitative real-time PCR (qPCR). <i>hsa-miR-181a-5p</i> was downregulated and associated with increased MS risk (<i>P</i> = 0.012). The other three miRNAs were upregulated and not associated with MS (<i>P</i> < 0.05). The area under the curve (AUC) is 0.779. In silico analyses showed that <i>hsa-miR-181a-5p</i> may participate in MS pathology by targeting <i>MAP2K1</i>, <i>CREB1</i>, <i>ATXN1</i>, and <i>ATXN3</i> genes in inflammation and neurodegeneration pathways. The circulatory <i>hsa-miR-181a-5p</i> can regulate target genes, reversing the mechanisms involved in MS pathologies such as protein uptake and processing, cell proliferation and survival, inflammation, and neurodegeneration. Thus, this miRNA could be used as an epigenomic-guided diagnostic tool and for therapeutic purpose.</p>","PeriodicalId":12809,"journal":{"name":"Genome","volume":"65 11","pages":"547-561"},"PeriodicalIF":2.3000,"publicationDate":"2022-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"3","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Genome","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1139/gen-2022-0040","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2022/9/14 0:00:00","PubModel":"Epub","JCR":"Q3","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
引用次数: 3

Abstract

Multiple sclerosis (MS) is a chronic inflammatory disease of the central nervous system (CNS) characterized by demyelination and axonal degeneration. Abnormal expression of microRNAs (miRNAs) plays an important role in MS pathology. In this cohort study, differential expression of the four miRNAs (hsa-miR-155-5phsa-miR-9-5phsa-miR-181a-5p, and hsa-miR-125b-5p) was investigated in 69 individuals, including 39 MS patients (relapsing-remitting MS (RRMS), n = 27; secondary progressive MS (SPMS), n = 12) and 30 healthy controls. In silico analyses revealed possible genes and pathways specific to miRNAs. Peripheral blood miRNA expressions were detected by quantitative real-time PCR (qPCR). hsa-miR-181a-5p was downregulated and associated with increased MS risk (P = 0.012). The other three miRNAs were upregulated and not associated with MS (P < 0.05). The area under the curve (AUC) is 0.779. In silico analyses showed that hsa-miR-181a-5p may participate in MS pathology by targeting MAP2K1CREB1ATXN1, and ATXN3 genes in inflammation and neurodegeneration pathways. The circulatory hsa-miR-181a-5p can regulate target genes, reversing the mechanisms involved in MS pathologies such as protein uptake and processing, cell proliferation and survival, inflammation, and neurodegeneration. Thus, this miRNA could be used as an epigenomic-guided diagnostic tool and for therapeutic purpose.

miR-181a-5p是多发性硬化症的潜在候选表观遗传生物标志物。
多发性硬化(MS)是一种以脱髓鞘和轴突变性为特征的中枢神经系统(CNS)慢性炎症性疾病。microRNAs (miRNAs)的异常表达在MS病理中起着重要作用。在这项队列研究中,研究人员在69名个体中研究了四种mirna (hsa-miR-155-5p、hsa-miR-9-5p、hsa-miR-181a-5p和hsa-miR-125b-5p)的差异表达,其中包括39名MS患者(复发-缓解型MS (RRMS), n = 27;继发性进展性MS (SPMS, n = 12)和30名健康对照。计算机分析揭示了可能的mirna特异性基因和途径。采用实时荧光定量PCR (qPCR)检测外周血miRNA的表达。hsa-miR-181a-5p下调并与MS风险增加相关(P = 0.012)。其他三种mirna上调,与MS无关(P hsa-miR-181a-5p可能通过靶向炎症和神经退行性通路中的MAP2K1、CREB1、ATXN1和ATXN3基因参与MS病理。循环hsa-miR-181a-5p可以调节靶基因,逆转MS病理如蛋白质摄取和加工、细胞增殖和存活、炎症和神经变性等机制。因此,该miRNA可作为表观基因组指导的诊断工具和治疗目的。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Genome
Genome 生物-生物工程与应用微生物
CiteScore
5.30
自引率
3.20%
发文量
42
审稿时长
6-12 weeks
期刊介绍: Genome is a monthly journal, established in 1959, that publishes original research articles, reviews, mini-reviews, current opinions, and commentaries. Areas of interest include general genetics and genomics, cytogenetics, molecular and evolutionary genetics, developmental genetics, population genetics, phylogenomics, molecular identification, as well as emerging areas such as ecological, comparative, and functional genomics.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信