{"title":"Immunohistochemistry.","authors":"Patricia A Loughran, Mark A Ross, Claudette M St Croix","doi":"10.1002/cpz1.549","DOIUrl":null,"url":null,"abstract":"<p><p>Immunohistochemistry is an essential technique for the localization and measurement of proteins in cells and tissues. This article describes methods for labeling proteins in adherent and suspension cell cultures and in tissue sections. Choices of antibodies and detection methods are discussed, and detailed troubleshooting guidelines are provided. © 2022 Wiley Periodicals LLC. Basic Protocol 1: Immunofluorescent labeling of cells grown as adherent monolayers Alternate Protocol 1: Immunofluorescent labeling of cells in suspension Basic Protocol 2: Immunofluorescent labeling of tissue sections Alternate Protocol 2: Immunofluorescent labeling using streptavidin-biotin conjugates Alternate Protocol 3: Immunofluorescent double-labeling of tissue sections Alternate Protocol 4: Immunofluorescent double-labeling of tissue sections with two primary antibodies from the same host species.</p>","PeriodicalId":11174,"journal":{"name":"Current Protocols","volume":" ","pages":"e549"},"PeriodicalIF":0.0000,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"2","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Current Protocols","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1002/cpz1.549","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 2
Abstract
Immunohistochemistry is an essential technique for the localization and measurement of proteins in cells and tissues. This article describes methods for labeling proteins in adherent and suspension cell cultures and in tissue sections. Choices of antibodies and detection methods are discussed, and detailed troubleshooting guidelines are provided. © 2022 Wiley Periodicals LLC. Basic Protocol 1: Immunofluorescent labeling of cells grown as adherent monolayers Alternate Protocol 1: Immunofluorescent labeling of cells in suspension Basic Protocol 2: Immunofluorescent labeling of tissue sections Alternate Protocol 2: Immunofluorescent labeling using streptavidin-biotin conjugates Alternate Protocol 3: Immunofluorescent double-labeling of tissue sections Alternate Protocol 4: Immunofluorescent double-labeling of tissue sections with two primary antibodies from the same host species.
免疫组织化学。
免疫组织化学是细胞和组织中蛋白质定位和测量的重要技术。本文描述了贴壁和悬浮细胞培养和组织切片中标记蛋白质的方法。讨论了抗体的选择和检测方法，并提供了详细的故障排除指南。©2022 Wiley期刊有限责任公司基本方案1:作为贴壁单层生长的细胞的免疫荧光标记备用方案1:悬浮细胞的免疫荧光标记基本方案2:组织切片的免疫荧光标记备用方案2:使用链霉亲和素-生物素偶联物的免疫荧光标记备用方案3:组织切片的免疫荧光双标记备用方案4:用来自同一宿主物种的两种一抗对组织切片进行免疫荧光双重标记。
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