Effects of Oakmoss Components on Extra- and Intracellular Legionella pneumophila and Its Host Acanthamoeba castellanii.

IF 0.9 4区 农林科学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Harue Nomura, Nobumasa Suda, Yasuyo Kawano, Yasunori Isshiki, Keisuke Sakuda, Katsuya Sakuma, Seiichi Kondo
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引用次数: 1

Abstract

Acanthamoeba castellanii is a ubiquitous organism found in environmental water. The amoeba is pathogenic to toward humans and is also a reservoir of bacteria of the genus Legionella, a causative agent of legionellosis. Oakmoss, a source of natural fragrance ingredients, and its components are antibacterial agents that are specifically active against the genus Legionella. In the present study, oakmoss and its components were investigated for their inhibitory effects on total (extra- and intracellular) Legionella pneumophila within A. castellanii and on L. pneumophila within A. castellanii. Among the oakmoss components, 3-hydroxy-5-methylphenyl 2,4-dihydroxy-6-methylbenzoate (1), 3-methoxy-5-methylphenyl 2,4-dihydroxy-6-methylbenzoate (2), and 8-(2,4-dihydroxy-6-(2-oxoheptyl)phenoxy)-6-hydroxy-3-pentyl-1H-isochromen-1-one (8) reduced the number of total bacteria (extra- and intracellular) in a test culture and also exhibited high amoebicidal activity against L. pneumophila within A. castellanii at concentrations lower than their IC50 values for A. castellanii. In contrast, 6,8-dihydroxy-3-pentyl-1H-isochromen-1-one (5) reduced the total number of L. pneumophila and, also that of total bacteria after 24 h of treatment (P < 0.05), whereas the compound did not exhibit amoebicidal activity against L. pneumophila within A. castellanii at concentrations lower than its IC50 value against A. castellanii. Thus, it is suggested that these oakmoss components could be good candidates for disinfectants to protect from Legionella infection.

橡苔成分对嗜肺军团菌胞外和胞内及其宿主棘阿米巴的影响。
棘阿米巴是一种普遍存在于环境水体中的生物。变形虫对人类具有致病性,也是军团菌属细菌的储存库,军团菌属细菌是军团菌病的病原体。橡苔是天然香料成分的来源,其成分是抗菌剂,对军团菌属有特别的活性。本研究研究了橡苔及其组分对castellanii嗜肺军团菌(胞外和胞内)和castellanii嗜肺军团菌的抑制作用。在栎苔组分中,3-羟基-5-甲基苯基2,4-二羟基-6-甲基苯甲酸酯(1)、3-甲氧基-5-甲基苯基2,4-二羟基-6-甲基苯甲酸酯(2)和8-(2,4-二羟基-6-(2-氧庚基)苯氧基)-6-羟基-3-戊基-1-异色门-1- 1(8)在低于其对castellanii IC50值的浓度下,可减少试验培养中细菌总数(细胞外和细胞内),并对castellanii嗜肺乳杆菌具有较高的杀阿米巴活性。相比之下,6,8-二羟基-3-戊基- 1h -等色门-1- 1(5)在处理24 h后减少了嗜肺乳杆菌的总数和总细菌数(P < 0.05),而在浓度低于其对castellanii的IC50值时,该化合物对castellanii内的嗜肺乳杆菌没有杀阿米巴活性。因此,这些橡树苔成分可能是预防军团菌感染的良好候选消毒剂。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Biocontrol science
Biocontrol science BIOTECHNOLOGY & APPLIED MICROBIOLOGY-
CiteScore
2.60
自引率
8.30%
发文量
21
审稿时长
>12 weeks
期刊介绍: The Biocontrol Science provides a medium for the publication of original articles, concise notes, and review articles on all aspects of science and technology of biocontrol.
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