Comparative Study to Assess the Effectiveness of Various Disinfecta- nts on two Microorganisms and the effect of same on Flexural Strength of Acrylic Denture Base Resin - An In Vitro Study.
S Ganesh, Anil Kumar Gujjari, Shylesh Kumar B S, Ravi M B, Sowmya S, Meenakshi S
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Abstract
Background: To evaluate and compare the effectiveness of various disinfectants on Candida albicans (C.albicans) and Staphylococcus aureus (Staph.aureus) inoculated on acrylic denture base resin and effect of disinfectants on flexural strength of denture base resin.
Materials & methods: A total of 130 acrylic denture base resin specimens were fabricated and processed according to manufacturer instructions. 82 sterile specimens were used for microbiological study. 2 specimens were cultured for organism growth to ensure sterility. 40 sterile specimens each were inoculated by immersing in Sabouraud & Nutrient broth containing microorganisms for 45 minutes each. Then the specimens were immersed in chlorhexidine, glutaraldehyde & distilled water (control) for 4 & 8 minutes. Then the specimens were neutralized. After neutralization the specimens were cultured onto Sabouraud's broth for C.albicans and Nutrient broth for Staph.aureus incubated for 72 h and observed for turbidity. At the end of 72 h subculture were made onto Sabourads dextrose agar media for C.albicans, Blood agar media for Staph.aureus and incubated for 48 h to observe growth. For flexural strength testing, 8 specimens each was immersed in the above mentioned disinfectants and distilled water for 8 & 16 minutes. Each of which was then subjected to 3 point flexural load in Lloyd's Universal testing machine. The peak load was recorded and flexural strength values were calculated.
Results: The microbiological study revealed that both disinfectants were equally effective at 4 minutes against C.albicans & Staph.aureus microorganisms. Flexural strength test revealed no significant difference between test and control groups.
Conclusion: Chlorhexdine and Glutaraldehyde disinfectans are equally effective against C.albicans and Staph.aureus microorganisms. Heat polymerized acrylic denture base resin did not demonstrate any significant change in flexural strength between control and test specimens. How to cite this article: Ganesh S, Gujjari A K, Shylesh K B S, Ravi M B, Sowmya S, Meenakshi S.Comparative Study to Assess the Effectiveness of Various Disinfectants on two Microorganisms and the effect of same on Flexural Strength of Acrylic Denture Base Resin - An In Vitro Study. J Int Oral Health 2013; 5(3):55-62.
背景:评价和比较不同消毒剂对接种在丙烯酸义齿基托树脂上的白色念珠菌(c.b albicans)和金黄色葡萄球菌(Staphylococcus aureus)的效果及消毒剂对义齿基托树脂弯曲强度的影响。材料与方法:根据生产厂家的说明书,制作并加工共130个丙烯酸基托树脂样品。无菌标本82份进行微生物学研究。2个标本培养用于生物生长,以确保无菌。将40份无菌标本分别接种于含微生物的Sabouraud & nutrition肉汤中,浸泡45分钟。然后将标本浸泡在氯己定、戊二醛和蒸馏水(对照)中4和8分钟。然后将标本中和。中和后的标本分别在Sabouraud培养液中培养白色念珠菌,在营养培养液中培养葡萄球菌。金黄色葡萄培养72小时,观察浑浊度。72h后在Sabourads葡萄糖琼脂培养基上传代培养白色念珠菌,在血琼脂培养基上传代培养葡萄球菌。培养48小时观察生长情况。抗弯强度试验将8个试样分别浸泡在上述消毒剂和蒸馏水中8和16分钟。然后在劳合社通用试验机中对每一个进行三点弯曲载荷测试。记录峰值荷载并计算抗弯强度值。结果:微生物学研究表明,这两种消毒剂在4分钟内对白色念珠菌和葡萄球菌同样有效。球菌微生物。抗折强度试验结果显示,试验组与对照组无显著差异。结论:氯己定与戊二醛消毒液对白色念珠菌和葡萄球菌具有相同的抑菌效果。球菌微生物。热聚合丙烯酸义齿基托树脂在对照和试验样品之间的抗弯强度没有明显变化。ganesesh S, Gujjari A K, Shylesh K B S, Ravi M B, Sowmya S, Meenakshi .不同消毒剂对两种微生物的效果及其对丙烯酸义齿基托树脂抗弯强度的影响的体外研究。国际口腔卫生杂志;2013;5(3): 55 - 62。