Downregulation of miR-145 expression in oral squamous cell carcinomas and its clinical significance.

IF 0.2 4区医学 Q4 Medicine

Onkologie Pub Date : 2013-01-01 Epub Date: 2013-03-18 DOI:10.1159/000349956

Ling Gao, Wenhao Ren, Su'e Chang, Bo Guo, Shuo Huang, Menghe Li, Youmin Guo, Zongfang Li, Tusheng Song, Keqian Zhi, Chen Huang

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引用次数: 15

Abstract

Background: MicroRNAs have been reported to play roles as oncogenes or tumor suppressor genes in human cancers. However, the expression levels of miR-145 in oral squamous cell carcinoma (OSCC) are unclear. The purpose of this study was to investigate the status of miR-145 expression in OSCC and determine its clinical significance.

Patients and methods: We examined miR-145 levels in 62 OSCC tissue samples and cell lines by quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR). The relationship between miR-145 expression and clinicopathologic factors of OSCC patients was analyzed.

Results: The proportion of miR-145 low expression was 82.26% (51/62) among the 62 OSCC patients, and expression levels of miR-145 in OSCC tissue samples and cell lines were significantly lower than in non-tumor controls. miR-145 expression levels were not significantly associated with age (p = 0.607), sex (p = 0.213), location (p = 0.952), histology (p = 0.603), pT stage (p = 0.305), pTNM stage (p = 0.471), and lymphatic metastasis (p = 1.000).

Conclusion: miR-145 may be involved in the early tumorigenesis of oral squamous cells, and might be a potential biomarker in the early diagnosis of OSCC.

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miR-145在口腔鳞状细胞癌中的下调表达及其临床意义

背景:MicroRNAs已被报道在人类癌症中作为癌基因或肿瘤抑制基因发挥作用。然而，miR-145在口腔鳞癌(OSCC)中的表达水平尚不清楚。本研究的目的是探讨miR-145在OSCC中的表达状况，并确定其临床意义。患者和方法:我们通过定量实时逆转录聚合酶链反应(qRT-PCR)检测了62个OSCC组织样本和细胞系中miR-145的水平。分析miR-145表达与OSCC患者临床病理因素的关系。结果:62例OSCC患者中miR-145低表达比例为82.26%(51/62)，且miR-145在OSCC组织样本和细胞系中的表达水平明显低于非肿瘤对照组。miR-145表达水平与年龄(p = 0.607)、性别(p = 0.213)、部位(p = 0.952)、组织学(p = 0.603)、pT分期(p = 0.305)、pTNM分期(p = 0.471)、淋巴转移(p = 1.000)无显著相关性。结论:miR-145可能参与口腔鳞状细胞的早期肿瘤发生，可能是OSCC早期诊断的潜在生物标志物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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