Functions of the Hha and YdgT proteins in transcriptional silencing by the nucleoid proteins, H-NS and StpA, in Escherichia coli.

Takeshi Ueda, Hiroki Takahashi, Ebru Uyar, Shu Ishikawa, Naotake Ogasawara, Taku Oshima
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引用次数: 44

Abstract

The Hha and YdgT proteins are suggested to modulate the expression of horizontally acquired genes by interacting with H-NS and StpA, which play central roles in the transcriptional silencing of such genes. However, it is also possible that Hha/YdgT repress gene expression independently of H-NS/StpA, as we have not fully understood the molecular mechanism through which Hha/YdgT modulate H-NS/StpA activity. To gain further insight into the basic functions of Hha/YdgT, we analysed the impact of hha/ydgT double inactivation on the transcriptome profile of Escherichia coli K-12, and compared the effects with that of hns/stpA double inactivation. In addition, we examined the effects of hha/ydgT inactivation on the chromosomal binding of H-NS, and conversely the effects of hns/stpA inactivation on the chromosomal binding of Hha. Our results demonstrated that the chromosomal binding of Hha requires H-NS/StpA, and is necessary for the repression of a subset of genes in the H-NS/StpA regulon. Furthermore, the distribution of H-NS binding around Hha/YdgT-dependent and -independent genes suggests that Hha/YdgT proteins modulate formation of the H-NS/StpA-DNA complex.

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Hha和YdgT蛋白在大肠杆菌类核蛋白H-NS和StpA转录沉默中的作用
Hha和YdgT蛋白可能通过与H-NS和StpA相互作用来调节水平获得基因的表达,而H-NS和StpA在水平获得基因的转录沉默中起着核心作用。然而,也有可能Hha/YdgT独立于H-NS/StpA抑制基因表达,因为我们还没有完全了解Hha/YdgT调节H-NS/StpA活性的分子机制。为了进一步了解Hha/YdgT的基本功能,我们分析了Hha/YdgT双失活对大肠杆菌K-12转录组谱的影响,并与hns/stpA双失活的影响进行了比较。此外,我们还检测了hha/ydgT失活对H-NS染色体结合的影响,以及hns/stpA失活对hha染色体结合的影响。我们的研究结果表明,Hha的染色体结合需要H-NS/StpA,并且是抑制H-NS/StpA调控中的一部分基因所必需的。此外,Hha/YdgT依赖性和非依赖性基因周围H-NS结合的分布表明,Hha/YdgT蛋白调节H-NS/StpA-DNA复合物的形成。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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