Functional centromeres in Astragalus sinicus include a compact centromere-specific histone H3 and a 20-bp tandem repeat.

Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology Pub Date : 2011-11-01 Epub Date: 2011-11-08 DOI:10.1007/s10577-011-9247-y

Ahmet L Tek, Kazunari Kashihara, Minoru Murata, Kiyotaka Nagaki

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引用次数: 25

Abstract

The centromere plays an essential role for proper chromosome segregation during cell division and usually harbors long arrays of tandem repeated satellite DNA sequences. Although this function is conserved among eukaryotes, the sequences of centromeric DNA repeats are variable. Most of our understanding of functional centromeres, which are defined by localization of a centromere-specific histone H3 (CENH3) protein, comes from model organisms. The components of the functional centromere in legumes are poorly known. The genus Astragalus is a member of the legumes and bears the largest numbers of species among angiosperms. Therefore, we studied the components of centromeres in Astragalus sinicus. We identified the CenH3 homolog of A. sinicus, AsCenH3 that is the most compact in size among higher eukaryotes. A CENH3-based assay revealed the functional centromeric DNA sequences from A. sinicus, called CentAs. The CentAs repeat is localized in A. sinicus centromeres, and comprises an AT-rich tandem repeat with a monomer size of 20 nucleotides.

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黄芪的功能着丝粒包括一个致密的着丝粒特异性组蛋白H3和一个20 bp的串联重复序列。

着丝粒在细胞分裂过程中对正确的染色体分离起着至关重要的作用，通常含有长串重复卫星DNA序列。虽然这一功能在真核生物中是保守的，但着丝粒DNA重复序列是可变的。着丝粒是由着丝粒特异性组蛋白H3 (CENH3)蛋白的定位定义的，我们对功能着丝粒的理解大多来自模式生物。豆科植物中功能性着丝粒的组成成分所知甚少。黄芪属是豆科植物的一员，是被子植物中种类最多的。因此，我们对黄芪中着丝粒的组成进行了研究。我们鉴定了A. sinicus的CenH3同源物，AsCenH3是高等真核生物中体积最紧凑的。基于cenh3的分析揭示了sinicus的功能着丝粒DNA序列，称为CentAs。CentAs重复序列定位于a . sinicus着丝粒，包含一个富含at的串联重复序列，单体大小为20个核苷酸。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology

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