{"title":"A photochemically initiated chemistry for coupling underivatized carbohydrates to gold nanoparticles.","authors":"Xin Wang, Olof Ramström, Mingdi Yan","doi":"10.1039/B917900C","DOIUrl":null,"url":null,"abstract":"<p><p>The sensitive optoelectronic properties of metal nanoparticles make nanoparticle-based materials a powerful tool to study fundamental biorecognition processes. Here we present a new and versatile method for coupling underivatized carbohydrates to gold nanoparticles (Au NPs) via the photochemically induced reaction of perfluorophenylazide (PFPA). A one-pot procedure was developed where Au NPs were synthesized and functionalized with PFPA by a ligand-exchange reaction. Carbohydrates were subsequently immobilized on the NPs by a fast light activation. The coupling reaction was efficient, resulting in high coupling yield as well as high ligand surface coverage. A colorimetric system based on the carbohydrate-modified Au NPs was used for the sensitive detection of carbohydrate-protein interactions. Binding and cross-reactivity studies were carried out between carbohydrate-functionalized Au NPs and lectins. Results showed that the surface-bound carbohydrates not only retained their binding affinities towards the corresponding lectin, but also exhibited affinity ranking consistent with that of the free ligands in solution.</p>","PeriodicalId":16297,"journal":{"name":"Journal of Materials Chemistry","volume":"19 47","pages":"8944-8949"},"PeriodicalIF":0.0000,"publicationDate":"2009-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2941222/pdf/nihms174271.pdf","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Materials Chemistry","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1039/B917900C","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
The sensitive optoelectronic properties of metal nanoparticles make nanoparticle-based materials a powerful tool to study fundamental biorecognition processes. Here we present a new and versatile method for coupling underivatized carbohydrates to gold nanoparticles (Au NPs) via the photochemically induced reaction of perfluorophenylazide (PFPA). A one-pot procedure was developed where Au NPs were synthesized and functionalized with PFPA by a ligand-exchange reaction. Carbohydrates were subsequently immobilized on the NPs by a fast light activation. The coupling reaction was efficient, resulting in high coupling yield as well as high ligand surface coverage. A colorimetric system based on the carbohydrate-modified Au NPs was used for the sensitive detection of carbohydrate-protein interactions. Binding and cross-reactivity studies were carried out between carbohydrate-functionalized Au NPs and lectins. Results showed that the surface-bound carbohydrates not only retained their binding affinities towards the corresponding lectin, but also exhibited affinity ranking consistent with that of the free ligands in solution.
金属纳米粒子灵敏的光电特性使基于纳米粒子的材料成为研究基本生物识别过程的有力工具。在这里,我们介绍了一种通过全氟苯基叠氮化物(PFPA)的光化学诱导反应将未充分活化的碳水化合物与金纳米粒子(Au NPs)耦合的多功能新方法。我们开发了一种一锅程序,通过配体交换反应合成 Au NPs 并用 PFPA 进行功能化。随后通过快速光激活将碳水化合物固定在 NPs 上。耦合反应非常高效,不仅耦合产率高,而且配体表面覆盖率也很高。基于碳水化合物修饰的 Au NPs 的比色系统可用于灵敏检测碳水化合物与蛋白质之间的相互作用。在碳水化合物功能化 Au NPs 和凝集素之间进行了结合和交叉反应研究。结果表明,表面结合的碳水化合物不仅保持了与相应凝集素的结合亲和力,而且其亲和力等级与溶液中游离配体的亲和力等级一致。