IGF-1 knockdown inhibits phosphorylation of Akt and ERK in chicken embryonic myotubes

IF 1.6 4区医学 Q4 CELL BIOLOGY

Growth Hormone & Igf Research Pub Date : 2022-08-01 DOI:10.1016/j.ghir.2022.101478

Takaoki Saneyasu, Tomonori Nakamura, Kazuhisa Honda, Hiroshi Kamisoyama

引用次数: 1

Abstract

Objective

We examined whether auto/paracrine insulin-like growth factor-1 (IGF-1) contributes to the phosphorylation of Akt and ERK in chicken myotubes.

Methods

Chicken myotubes were treated with IGF-1 siRNA, and then total RNA and protein were harvested for real-time PCR and western blot analysis.

Results

Treatment with IGF-1 siRNA inhibited the phosphorylation of Akt and ERK, but not of ribosomal protein S6, in chicken myotubes. Interestingly, IGF-1 siRNA downregulated the expression of IGF-2.

Conclusions

The results of this study suggest that auto/paracrine IGF-1 contributes to Akt and ERK phosphorylation in chicken myotubes.

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IGF-1敲低可抑制鸡胚肌管中Akt和ERK的磷酸化

目的探讨自身/旁分泌胰岛素样生长因子-1 (IGF-1)是否参与鸡肌管中Akt和ERK的磷酸化。方法用IGF-1 siRNA处理鸡肌管，提取总RNA和蛋白，进行实时荧光定量PCR和western blot分析。结果IGF-1 siRNA可抑制鸡肌管中Akt和ERK的磷酸化，但对核糖体蛋白S6的磷酸化无抑制作用。有趣的是，IGF-1 siRNA下调了IGF-2的表达。结论自分泌/旁分泌IGF-1参与鸡肌管中Akt和ERK的磷酸化。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Growth Hormone & Igf Research 医学-内分泌学与代谢

CiteScore

3.30

自引率

0.00%

发文量

审稿时长

57 days

期刊介绍： Growth Hormone & IGF Research is a forum for research on the regulation of growth and metabolism in humans, animals, tissues and cells. It publishes articles on all aspects of growth-promoting and growth-inhibiting hormones and factors, with particular emphasis on insulin-like growth factors (IGFs) and growth hormone. This reflects the increasing importance of growth hormone and IGFs in clinical medicine and in the treatment of diseases.