The species of Oxytropis DC. of section Gloeocephala Bunge (Fabaceae) from Northeast Asia: genetic diversity and relationships based on sequencing of the intergenic spacers of cpDNA and ITS nrDNA.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2022-04-01 Epub Date: 2022-02-25 DOI:10.1007/s10709-022-00152-y

Alla Kholina, Marina Kozyrenko, Elena Artyukova, Valentin Yakubov, Mariya Khoreva, Elena Andrianova, Olga Mochalova, Denis Sandanov

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引用次数: 2

Abstract

Phylogenetic relationships within Oxytropis DC. sect. Gloeocephala Bunge from Northeast Asia were studied using plastid intergenic spacers (psbA-trnH + trnL-trnF + trnS-trnG) and ITS nrDNA. Populations of O. anadyrensis Vass., O. borealis DC., O. middendorffii Trautv., O. trautvetteri Meinsh., and O. vasskovskyi Jurtz. were monomorphic or characterised by a low level of chloroplast genetic diversity (h varied from 0.143 to 0.692, and π from 0.0001 to 0.0005). Presumably, the low genetic diversity was a result of the severe bottlenecks during Pleistocene glaciation-interglacial cycles. Twenty chlorotypes were identified; species studied had no shared chlorotypes. Chlorotypes of O. anadyrensis, O. borealis, and O. middendorffii formed two lineages each, while the chlorotypes of O. trautvetteri and O. vasskovskyi formed one separate lineage each in the phylogenetic network. There were specific diagnostic markers of cpDNA in each lineage, excluding O. vasskovskyi. The presence of a species-specific diagnostic marker in O. trautvetteri and specific markers in two lineages of O. anadyrensis support circumscribing these taxa as independent species. Regarding ITS nrDNA polymorphism, five ribotypes were detected. The differences revealed in plastid and nuclear genomes of Oxytropis sect. Gloeocephala confirmed that the Asian sector of Megaberingia was the main centre of diversification of arctic legumes.

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棘豆属植物。基于cpDNA和ITS - nrDNA基因间间隔序列的遗传多样性和亲缘关系。

棘豆属植物的系统发育关系。采用psbA-trnH + trl - trnf + trnS-trnG的质体间间隔序列和ITS nrDNA对东北亚Gloeocephala Bunge组进行了研究。白僵草的居群。， O. borealis DC。O.米德尔多菲·特劳特。O.特洛特维特特里·梅什。和O. vasskovskyi Jurtz。单一性或叶绿体遗传多样性较低(h变化范围为0.143 ~ 0.692，π变化范围为0.0001 ~ 0.0005)。据推测，低遗传多样性是更新世冰期-间冰期严重瓶颈的结果。鉴定出20种叶绿体;所研究的物种没有共同的叶绿体。在系统发育网络中，O. anadyrensis、O. borealis和O. middendorffii各形成两个分支，O. trautvetteri和O. vasskovskyi各形成一个独立的分支。在每个谱系中都有特定的cpDNA诊断标记，除了O. vasskovskyi。在O. trautvetteri和O. anadyrensis的两个谱系中存在一个种特异性诊断标记，支持将这些分类群限定为独立的物种。在ITS nrDNA多态性方面，检测到5种核糖型。Gloeocephala棘豆属(Oxytropis)组质体和核基因组的差异证实了megeringia亚洲区是北极豆科植物多样化的主要中心。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.