From more testing to smart testing: data-guided SARS-CoV-2 testing choices, the Netherlands, May to September 2020.

IF 7.8
Janko van Beek, Zsofia Igloi, Timo Boelsums, Ewout Fanoy, Hannelore Gotz, Richard Molenkamp, Jeroen van Kampen, Corine GeurtsvanKessel, Annemiek A van der Eijk, David van de Vijver, Marion Koopmans
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引用次数: 6

Abstract

BackgroundSARS-CoV-2 RT-PCR assays are more sensitive than rapid antigen detection assays (RDT) and can detect viral RNA even after an individual is no longer infectious. RDT can reduce the time to test and the results might better correlate with infectiousness.AimWe assessed the ability of five RDT to identify infectious COVID-19 cases and systematically recorded the turnaround time of RT-PCR testing.MethodsSensitivity of RDT was determined using a serially diluted SARS-CoV-2 stock with known viral RNA concentration. The probability of detecting infectious virus at a given viral load was calculated using logistic regression of viral RNA concentration and matched culture results of 78 specimens from randomly selected non-hospitalised cases. The probability of each RDT to detect infectious cases was calculated as the sum of the projected probabilities for viral isolation success for every viral RNA load found at the time of diagnosis in 1,739 confirmed non-hospitalised COVID-19 cases.ResultsThe distribution of quantification cycle values and estimated RNA loads for patients reporting to drive-through testing was skewed to high RNA loads. With the most sensitive RDT (Abbott and SD Biosensor), 97.30% (range: 88.65-99.77) of infectious individuals would be detected. This decreased to 92.73% (range: 60.30-99.77) for Coris BioConcept and GenBody, and 75.53% (range: 17.55-99.77) for RapiGEN. Only 32.9% of RT-PCR results were available on the same day as specimen collection.ConclusionThe most sensitive RDT detected infectious COVID-19 cases with high sensitivity and may considerably improve containment through more rapid isolation and contact tracing.

Abstract Image

Abstract Image

从更多的测试到智能测试:数据指导的SARS-CoV-2测试选择,荷兰,2020年5月至9月。
背景sars - cov -2 RT-PCR检测比快速抗原检测(RDT)更敏感,即使在个体不再具有传染性后也能检测到病毒RNA。RDT可以减少检测时间,结果可能更好地与传染性相关联。我们评估了5种RDT识别传染性COVID-19病例的能力,并系统记录了RT-PCR检测的周转时间。方法采用已知病毒RNA浓度的连续稀释SARS-CoV-2原液检测RDT的灵敏度。在给定病毒载量下检测感染性病毒的概率是通过对随机选择的非住院病例的78个标本的病毒RNA浓度和匹配培养结果进行逻辑回归来计算的。每次RDT检测感染病例的概率计算为1739例确诊非住院COVID-19病例中诊断时发现的每种病毒RNA载量的病毒分离成功预测概率的总和。结果报告驾车通过检测的患者的定量周期值和估计RNA负荷的分布偏向于高RNA负荷。最灵敏的RDT(雅培和SD Biosensor)检测阳性率为97.30%(范围:88.65 ~ 99.77)。Coris BioConcept和GenBody的这一比例降至92.73%(范围:60.30-99.77),RapiGEN为75.53%(范围:17.55-99.77)。只有32.9%的RT-PCR结果在标本采集当天可用。结论最灵敏的RDT检测出传染性COVID-19病例具有较高的敏感性,可以通过更快速的隔离和接触者追踪来显著提高防控效果。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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