A hyaluronic acid/PVA electrospun coating on 3D printed PLA scaffold for orthopedic application.

IF 4.4 3区 医学 Q2 ENGINEERING, BIOMEDICAL
Progress in Biomaterials Pub Date : 2022-03-01 Epub Date: 2022-01-22 DOI:10.1007/s40204-022-00180-z
Mina Farsi, Azadeh Asefnejad, Hadi Baharifar
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引用次数: 14

Abstract

The need for bone tissue replacement, repair and regeneration for orthopedic application is constantly growing. Therefore, the application of cartilage substitute due to the lack of donors as well as biocompatibility leads to immune system rejection. In order to overcome these drawbacks, researchers have used porous scaffold as an option for bone transplantation. In this study, poly-lactic acid (PLA) scaffolds were prepared for cartilage application by fused deposition modeling (FDM) technique and then coated by electrospinning with polyvinyl alcohol (PVA) and hyaluronic acid (HLA) fibers. Hybrid electrospinning (ELS) method was used to produce porous scaffolds from HLA-PVA polymers. The printed scaffold was coated using FDM technique and the mechanical and biological investigation was performed on the polymeric composite specimen. The functional group and morphological behavior were investigated using Fourier-transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM) techniques. The obtained porous scaffold has hydrophilic properties as the PVA and HLA were coated on the PLA. The porous 3D-printed scaffold containing PLA/PVA/HLA scaffold does not show any toxicity in MTT evaluation after 1, 3 and 7 days. The SEM image confirmed the cell adhesion of the chondrite to the scaffold. Also, the mechanical performances of the sample, such as elastic modulus and compressive strength, were evaluated by compression test. By electro-spun coating, the elastic module of PVA/PLA and PLA/PVA/HLA scaffolds has increased to 18.31 ± 0.29 MPa and 19.25 ± 0.38 MPa. Also, the tensile strength of these two porous scaffolds has reached 6.11 ± 0.42 MPa and 6.56 ± 0.14 MPa, respectively. The failure strain of 3D printed PLA scaffold was reported to be 53 ± 0.21% and this value was reduced to 47 ± 0.62% and 42 ± 0.22% in PVA/PLA and PLA/PVA/HLA scaffolds. The cells' growth on the porous scaffolds showed a broad, spindle-shaped and regular shape. The obtained results of the chemical, physical and biological analyses showed that porous PLA/PVA/HLA scaffold has potential applications in cartilage construction.

Abstract Image

用于骨科3D打印PLA支架的透明质酸/PVA电纺丝涂层。
骨科应用对骨组织置换、修复和再生的需求不断增长。因此,由于缺乏供体和生物相容性,软骨替代物的应用会导致免疫系统排斥。为了克服这些缺点,研究人员将多孔支架作为骨移植的一种选择。本研究采用熔融沉积建模(FDM)技术制备了用于软骨应用的聚乳酸(PLA)支架,并用聚乙烯醇(PVA)和透明质酸(HLA)纤维进行静电纺丝包覆。采用复合静电纺丝(ELS)法制备了HLA-PVA聚合物多孔支架。采用FDM技术对打印支架进行涂层处理,并对聚合物复合材料样品进行力学和生物学研究。利用傅里叶变换红外光谱(FTIR)和扫描电镜(SEM)技术研究了其官能团和形态行为。聚乙烯醇和HLA包被在聚乳酸表面后,所制备的多孔支架具有亲水性。含PLA/PVA/HLA支架的多孔3d打印支架在1、3、7天后的MTT评估中均未显示出任何毒性。扫描电镜图像证实了细胞粘附在支架上的球粒陨石。并通过压缩试验对试样的弹性模量、抗压强度等力学性能进行了评价。电纺丝涂层使PVA/PLA和PLA/PVA/HLA支架的弹性模量分别提高到18.31±0.29 MPa和19.25±0.38 MPa。两种多孔支架的抗拉强度分别达到6.11±0.42 MPa和6.56±0.14 MPa。3D打印PLA支架的失效应变为53±0.21%,而PVA/PLA和PLA/PVA/HLA支架的失效应变分别为47±0.62%和42±0.22%。细胞在多孔支架上的生长呈宽、梭形、规则的形态。化学、物理和生物分析结果表明,多孔PLA/PVA/HLA支架在软骨构建中具有潜在的应用前景。
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来源期刊
Progress in Biomaterials
Progress in Biomaterials MATERIALS SCIENCE, BIOMATERIALS-
CiteScore
9.60
自引率
4.10%
发文量
35
期刊介绍: Progress in Biomaterials is a multidisciplinary, English-language publication of original contributions and reviews concerning studies of the preparation, performance and evaluation of biomaterials; the chemical, physical, biological and mechanical behavior of materials both in vitro and in vivo in areas such as tissue engineering and regenerative medicine, drug delivery and implants where biomaterials play a significant role. Including all areas of: design; preparation; performance and evaluation of nano- and biomaterials in tissue engineering; drug delivery systems; regenerative medicine; implantable medical devices; interaction of cells/stem cells on biomaterials and related applications.
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