Screening for performance enhancing substances and quantification of ethanol in different Arishta manufactured in Sri Lanka.

IF 0.3 Q3 MEDICINE, GENERAL & INTERNAL

Ceylon Medical Journal Pub Date : 2020-12-31 DOI:10.4038/cmj.v65i4.9282

Nilu Fernando, Shehani Pigera, Nimesha Rashani, Ravindra Fernando, Pabasara Weerasinghe, Deepal Godakumbura, Madunil Niriella, Seevali Jayawickreme, Arjuna De Silva

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引用次数: 0

Abstract

Background: Arishta have been used in Ayurveda medicine for over thousands of years in Sri Lanka to treat various diseases. Ashwagandharishta, Balarishta and Dashamoolarishta are usually prescribed to obtain an anabolic effect, and Ashwagandharishta and Dashamoolarishta for androgenic effect in males. Thus, these arishta have been shown to have similar effect as anabolic androgenic steroids and stimulants in Western medicine. Therefore, arishta could potentially be used by athletes to improve their performance in sports leading to unintentional doping. Additionally, ethanol develops in-source during arista fermentation, which can affect athletes health.

Objective: The aim of this study is to investigate whether the anabolic androgenic steroids or stimulants banned by World Anti-Doping Agency are present in these arishta, and to determine their ethanol content.

Methods: Methanol extractions of Ashwagandarishta, Balarishta, Dashamoolarishta from four different manufacturers were screened for 21 stimulant and 22 anabolic androgenic steroids banned by World Anti-Doping Agency, using Gas Chromatography Mass Spectrometer. Ethanol content of the twelve Arishta samples were also measured.

Results: Anabolic androgenic steroids or stimulants were not present in the tested Arishta samples, and percentage volume / volume ethanol content of all Arishta was between (5.80-8.35) ±0.5.

Conclusion: The tested brands of Ashwagandharishta, Balarishta and Dashamoolarishta did not contain stimulants or anabolic androgenic steroids banned by World Anti-Doping Agency.

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斯里兰卡产阿利什塔中性能增强物质的筛选及乙醇的定量分析。

背景:在斯里兰卡，阿育吠陀医学中使用阿里什塔已经有几千年的历史了，用来治疗各种疾病。Ashwagandharishta, Balarishta和Dashamoolarishta通常用于获得合成代谢效果，而Ashwagandharishta和Dashamoolarishta用于男性的雄激素作用。因此，这些arishta已被证明具有与西方医学中合成代谢雄激素类固醇和兴奋剂相似的效果。因此，arishta可能会被运动员用来提高他们在运动中的表现，从而导致无意中服用兴奋剂。此外，乙醇在马兜草发酵过程中产生，这可能会影响运动员的健康。目的:本研究的目的是调查这些阿利什塔中是否含有合成代谢雄激素类固醇或世界反兴奋剂机构禁用的兴奋剂，并测定其乙醇含量。方法:采用气相色谱-质谱联用技术，对4家不同生产厂家的Ashwagandarishta、Balarishta、Dashamoolarishta的甲醇提取物中21种兴奋剂和22种世界反兴奋剂机构禁用的合成代谢雄激素进行筛选。测定了12种Arishta样品的乙醇含量。结果:在所检测的Arishta样品中不存在合成代谢雄激素类固醇或兴奋剂，所有Arishta的体积/体积乙醇含量在(5.80-8.35)±0.5之间。结论:检测品牌“阿什瓦甘达里希塔”、“巴拉里希塔”和“达沙莫拉里希塔”不含世界反兴奋剂机构禁用的兴奋剂和合成代谢雄激素类固醇。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Ceylon Medical Journal MEDICINE, GENERAL & INTERNAL-

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期刊介绍： The Ceylon Medical Journal, is the oldest surviving medical journal in Australasia. It is the only medical journal in Sri Lanka that is listed in the Index Medicus. The CMJ started life way back in 1887 as the organ of the Ceylon Branch of the British Medical Association. Except for a brief period between 1893 and 1904 when it ceased publication, the CMJ or its forbear, the Journal of the Ceylon Branch of the British Medical Association, has been published without interruption up to now. The journal"s name changed to the CMJ in 1954.