The Hedgehog pathway in penaeid shrimp: developmental expression and evolution of splice junctions in Pancrustacea.

IF 1.3 4区 生物学 Q4 GENETICS & HEREDITY
Genetica Pub Date : 2022-04-01 Epub Date: 2022-02-07 DOI:10.1007/s10709-022-00151-z
Philip L Hertzler, Emma J Devries, Rachel A DeBoer
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引用次数: 2

Abstract

Penaeid shrimp embryos undergo holoblastic division, gastrulation by invagination, and hatching as a nauplius larva. Posterior segments form and differentiate during larval development. Hedgehog (Hh) pathway genes from penaeid shrimp and other pancrustaceans were identified by in silico analysis of genomes and transcriptomes, and mapped onto a recent pancrustacean phylogeny to determine patterns of intron gains and losses. Penaeus vannamei, P. japonicus, and P. monodon Hh proteins were encoded by four exons. Amphipod, isopod, and ostracod hh were also encoded by four exons, but hh from other arthropod groups contained three conserved exons. The novel hh intron is hypothesized to have arisen independently in the malacostracan ancestor and Ostracoda by a transposon insertion. Shared patterns of ptc, smo, and ci exon structure were found for Malacostraca, Branchiopoda + Hexapoda, Hexanauplia (Thecostraca + Copepoda), Multicrustacea (Thecostraca + Copepoda + Malacostraca), and Pancrustacea minus Oligostraca. mRNA expression of P. vannamei of hh, ptc, and ci from developmental transcriptomes of zygotes through postlarvae showed low expression from zygote to gastrula, which increased at limb bud, peaked at unhatched nauplius, and declined in nauplius and later larval stages. smo expression was found in zygotes, peaked in gastrula, and declined in limb bud and later stages. These results are consistent with a role for Hh signaling during segmentation in penaeid shrimp.

对虾的Hedgehog通路:平壳类动物剪接连接的发育表达和进化。
对虾胚经历成全细胞分裂,内陷形成原肠胚,并孵化成无钩体幼体。后节在幼虫发育过程中形成和分化。通过基因组和转录组的计算机分析,从对虾和其他潘壳纲动物中鉴定出Hedgehog (Hh)通路基因,并将其映射到最近的潘壳纲动物系统发育中,以确定内含子的获得和损失模式。凡纳滨对虾、日本对虾和单对虾Hh蛋白由4个外显子编码。片足类、等足类和介形虫类的hh也由4个外显子编码,但其他节肢动物类群的hh包含3个保守外显子。新的hh内含子被假设是通过转座子插入在甲壳目和介形虫目中独立出现的。Malacostraca、Branchiopoda +六足目、Hexanauplia (the ecostraca + Copepoda)、multicrustaceae (the ecostraca + Copepoda + Malacostraca)和panrustaceae - Oligostraca的ptc、smo和ci外显子结构具有共同的模式。从受精卵发育转录组中,凡纳滨扁豆hh、ptc和ci mRNA的表达从受精卵到原肠胚阶段均表现为低表达,在肢芽阶段表达量增加,在未孵化幼体阶段达到峰值,在幼体及后期幼虫阶段表达量下降。Smo在受精卵中表达,在原肠胚中表达高峰,在肢芽及后期表达下降。这些结果与Hh信号在对虾分割过程中的作用一致。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Genetica
Genetica 生物-遗传学
CiteScore
2.70
自引率
0.00%
发文量
32
审稿时长
>12 weeks
期刊介绍: Genetica publishes papers dealing with genetics, genomics, and evolution. Our journal covers novel advances in the fields of genomics, conservation genetics, genotype-phenotype interactions, evo-devo, population and quantitative genetics, and biodiversity. Genetica publishes original research articles addressing novel conceptual, experimental, and theoretical issues in these areas, whatever the taxon considered. Biomedical papers and papers on breeding animal and plant genetics are not within the scope of Genetica, unless framed in an evolutionary context. Recent advances in genetics, genomics and evolution are also published in thematic issues and synthesis papers published by experts in the field.
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