Increased lacrimal inflammatory mediators in patients with keratoconus.

IF 1.8 3区医学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Molecular Vision Pub Date : 2021-12-07 eCollection Date: 2021-01-01

Gustavo Souza Moura, Albert Santos, Marcos Antonio Cenedeze, Meire Ioshie Hiyane, Niels Olsen Saraiva Camara, Luciene Barbosa de Sousa, Lauro Augusto de Oliveira

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引用次数: 0

Abstract

Purpose: This study aimed to characterize the tear film immunologic profile in keratoconus (KC) patients compared with healthy individuals (control group) and to investigate the correlation between the tear film immunologic profile and atopy, disease severity, and disease status over time.

Methods: The study involved 30 KC patients and 18 healthy individuals. Tear collection was obtained using microcapillary tubes. Tear film levels of fractalkine, granulocyte-macrophage colony-stimulating factor (GM-CSF), interferon (IFN)-γ, interleukin (IL)-1β, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-10, IL-12, IL-13, IL-17A, IL-21, IL-23, interferon-inducible T-cell alpha chemoattractant (ITAC), macrophage inflammatory protein-1 alpha (MIP-1α), MIP-1β, MIP-3α, and tumor necrosis factor (TNF)-α were detected. Keratometric measurements and topographic patterns were used to diagnose and define disease progression. Tear immunologic profiles were compared, emphasizing the presence or absence of ocular allergy. Correlations between the cytokine profile, disease severity, and disease status were also analyzed longitudinally in the KC patients.

Results: Lacrimal cytokine concentrations were higher in the KC patients than they were in the controls in 14 of 21 cytokines analyzed. IL-6 was the most relevant cytokine found in KC patients, especially when associated with ocular allergy. There was no correlation between KC progression and the level of inflammatory cytokines when analyzed longitudinally. KC severity correlated with IL-6 concentration, where the more severe KC presented a higher IL-6 concentration in tears.

Conclusions: Inflammatory activity seems to be involved in the pathogenesis of KC. Out of 21 cytokines, 14 were more concentrated in the tears of KC patients than healthy subjects. IL-6 was significantly higher in KC patients' tears and was related to disease severity. Disease progression did not correlate with cytokine levels when analyzed longitudinally.

Abstract Image

本刊更多论文

圆锥角膜患者泪道炎症介质增加。

目的:本研究旨在描述圆锥角膜(KC)患者与健康个体(对照组)的泪膜免疫特征，并探讨泪膜免疫特征与特应性、疾病严重程度和疾病状态之间的相关性。方法:研究对象为30例KC患者和18例健康人。采用微毛细管收集泪液。检测撕裂膜中裂裂素、粒细胞-巨噬细胞集落刺激因子(GM-CSF)、干扰素(IFN)-γ、白细胞介素(IL)-1β、IL-2、IL-4、IL-5、IL-6、IL-7、IL-8、IL-10、IL-12、IL-13、IL- 17a、IL-21、IL-23、干扰素诱导的t细胞α趋化剂(ITAC)、巨噬细胞炎症蛋白-1α (MIP-1α)、MIP-1β、MIP-3α和肿瘤坏死因子(TNF)-α的水平。角膜测量和地形模式用于诊断和确定疾病进展。比较泪液免疫特征，强调眼部过敏的存在或不存在。在KC患者中，还纵向分析了细胞因子谱、疾病严重程度和疾病状态之间的相关性。结果:在分析的21种细胞因子中，KC患者泪道细胞因子浓度有14种高于对照组。IL-6是KC患者中发现的最相关的细胞因子，特别是与眼部过敏相关的细胞因子。当纵向分析时，KC进展与炎症细胞因子水平之间没有相关性。KC严重程度与IL-6浓度相关，KC越严重泪液中IL-6浓度越高。结论:炎性活动可能参与KC的发病机制，在21种细胞因子中，有14种细胞因子在KC患者的泪液中比健康人更集中。IL-6在KC患者泪液中显著升高，且与疾病严重程度相关。当纵向分析时，疾病进展与细胞因子水平无关。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Molecular Vision 生物-生化与分子生物学

CiteScore

4.40

自引率

0.00%

发文量

审稿时长

1 months

期刊介绍： Molecular Vision is a peer-reviewed journal dedicated to the dissemination of research results in molecular biology, cell biology, and the genetics of the visual system (ocular and cortical). Molecular Vision publishes articles presenting original research that has not previously been published and comprehensive articles reviewing the current status of a particular field or topic. Submissions to Molecular Vision are subjected to rigorous peer review. Molecular Vision does NOT publish preprints. For authors, Molecular Vision provides a rapid means of communicating important results. Access to Molecular Vision is free and unrestricted, allowing the widest possible audience for your article. Digital publishing allows you to use color images freely (and without fees). Additionally, you may publish animations, sounds, or other supplementary information that clarifies or supports your article. Each of the authors of an article may also list an electronic mail address (which will be updated upon request) to give interested readers easy access to authors.