Evaluation of Effects of Laboratory Disinfectants on Mouse Gut Microbiota.

IF 1.3 4区 农林科学 Q2 VETERINARY SCIENCES
Comparative medicine Pub Date : 2021-12-01 Epub Date: 2021-11-11 DOI:10.30802/AALAS-CM-21-000051
Joseph D Sciurba, George E Chlipala, Stefan J Green, Martha A Delaney, Jeffrey D Fortman, Jeanette E Purcell
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引用次数: 1

Abstract

Disturbances in the gut microbiota are known to be associated with numerous human diseases. Mice have proven to be an invaluable tool for investigating the role of the gut microbiota in disease processes. Nonexperimental factors related to maintaining mice in the laboratory environment are increasingly being shown to have inadvertent effects on the gut microbiota and may function as confounding variables. Microisolation technique is a term used to describe the common biosecurity practice of spraying gloved hands with disinfectant before handling research mice. This practice prevents contamination with pathogenic microorganisms. To investigate if exposure to disinfectants can affect the mouse gut microbiota, C57BL/6 mice were exposed daily for 27 consecutive days to commonly used laboratory disinfectants through microisolation technique. The effects of 70% ethanol and disinfectant products containing chlorine dioxide, hydrogen peroxide, or potassium peroxymonosulfate were each evaluated. Fecal pellets were collected after 7, 14, 21, and 28 d of disinfectant exposure, and cecal contents were collected at day 28. DNA extractions were performed on all cecal and fecal samples, and microbial community structure was characterized using 16S ribosomal RNA amplicon sequencing. Alpha and β diversity metrics and taxon-level analyses were used to evaluate differences in microbial communities. Disinfectant had a small but significant effect on fecal microbial communities compared with sham-exposed controls, and effects varied by disinfectant type. In general, longer exposure times resulted in greater changes in the fecal microbiota. Effects on the cecal microbiota were less pronounced and only seen with the hydrogen peroxide and potassium peroxymonosulfate disinfectants. These results indicate that laboratory disinfectant use should be considered as a potential factor that can affect the mouse gut microbiota.

实验室消毒剂对小鼠肠道菌群影响的评价。
众所周知,肠道菌群紊乱与许多人类疾病有关。小鼠已被证明是研究肠道微生物群在疾病过程中的作用的宝贵工具。与将小鼠维持在实验室环境中有关的非实验因素越来越多地被证明对肠道微生物群有无意的影响,并可能作为混杂变量发挥作用。微隔离技术是一个术语,用于描述在处理研究小鼠之前向戴着手套的手喷洒消毒剂的常见生物安全做法。这种做法可防止病原微生物污染。为了研究消毒剂暴露是否会影响小鼠肠道微生物群,采用微隔离技术,连续27天每天暴露于C57BL/6小鼠常用实验室消毒剂中。分别评价了70%乙醇和含有二氧化氯、过氧化氢或过氧单硫酸钾的消毒剂产品的效果。消毒后7、14、21、28 d收集粪便微球,28 d收集盲肠内容物。对所有盲肠和粪便样本进行DNA提取,并利用16S核糖体RNA扩增子测序对微生物群落结构进行表征。采用α和β多样性指标和分类群水平分析评价微生物群落差异。与假暴露对照组相比,消毒剂对粪便微生物群落的影响虽小但显著,且影响因消毒剂类型而异。一般来说,暴露时间越长,粪便微生物群的变化越大。对盲肠菌群的影响不太明显,只有过氧化氢和过氧单硫酸氢钾消毒剂才能看到。这些结果表明,实验室消毒剂的使用应被视为影响小鼠肠道微生物群的潜在因素。
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来源期刊
Comparative medicine
Comparative medicine 医学-动物学
CiteScore
1.90
自引率
0.00%
发文量
71
审稿时长
6-12 weeks
期刊介绍: Comparative Medicine (CM), an international journal of comparative and experimental medicine, is the leading English-language publication in the field and is ranked by the Science Citation Index in the upper third of all scientific journals. The mission of CM is to disseminate high-quality, peer-reviewed information that expands biomedical knowledge and promotes human and animal health through the study of laboratory animal disease, animal models of disease, and basic biologic mechanisms related to disease in people and animals.
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