Detection of Mycobacterium tuberculosis and Rifampicin Resistance Using GeneXpert MTB/RIF Assay at Enat Hospital, Central Ethiopia.

Tuberculosis Research and Treatment Pub Date : 2022-01-18 eCollection Date: 2022-01-01 DOI:10.1155/2022/1250404
Sebsib Selfegna, Amir Alelign
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引用次数: 6

Abstract

Background: Tuberculosis remains to be a public health threat in Ethiopia. However, the use of ill diagnostic methods and the lack of enough epidemiological information in the country contributed to the diagnostic delay and development of anti-TB drug resistance. Therefore, the present study is aimed at assessing the prevalence of pulmonary TB (PTB) and the development of drug resistance using GeneXpert MTB/RIF assay in Merhabete district, Central Ethiopia.

Methods: A cross-sectional, health facility-based study was conducted from December 2019 to June 2020. Bacteriological examination and GeneXpert molecular diagnostic methods were used for the detection of M. tuberculosis and rifampicin resistance (RR). Descriptive statistics and logistic regression analysis were used to determine the possible association of risk factors with the occurrence of PTB and RR. P values of <0.05 were considered statistically significant.

Results: The overall prevalence rates of PTB and RR M. tuberculosis were 11.2% and 15.8%, respectively. The logistic regression analysis revealed that being in the age group of 49-64 years was significantly associated with the occurrence of TB (P = 0.01). The odds of HIV-positive and retreatment study participants to be infected by M. tuberculosis were much more than those of HIV-negative and newly treated cases, respectively (P < 0.05). However, none of the sociodemographic and clinical patient characteristics was significantly associated with the development of RR-TB (P > 0.05).

Conclusion: In the present study, high prevalence rates of PTB and RR M. tuberculosis were observed. The findings, which were attributed to different risk factors, suggested an urgent need for appropriate intervention measures to reduce the transmission of PTB and the development of anti-TB drug resistance in the study area.

使用GeneXpert MTB/RIF检测埃塞俄比亚中部Enat医院结核分枝杆菌和利福平耐药性
背景:结核病在埃塞俄比亚仍然是一个公共卫生威胁。然而,在该国使用错误的诊断方法和缺乏足够的流行病学信息导致了诊断延误和抗结核药物耐药性的发展。因此,本研究旨在利用GeneXpert MTB/RIF测定法评估埃塞俄比亚中部Merhabete地区肺结核(PTB)的患病率和耐药性的发展。方法:2019年12月至2020年6月进行了一项基于卫生机构的横断面研究。采用细菌学检查和GeneXpert分子诊断方法检测结核分枝杆菌和利福平耐药性(RR)。采用描述性统计和logistic回归分析来确定危险因素与PTB发生和RR的可能关联。结果P值:PTB和RR M. tuberculosis的总患病率分别为11.2%和15.8%。logistic回归分析显示,49 ~ 64岁年龄组与TB的发生有显著相关性(P = 0.01)。hiv阳性和再治疗组感染结核分枝杆菌的几率明显高于hiv阴性组和新治疗组(P < 0.05)。然而,社会人口学和临床患者特征与RR-TB的发展均无显著相关性(P > 0.05)。结论:本研究区PTB和RR M. tuberculosis的患病率较高。这些发现归因于不同的危险因素,表明迫切需要采取适当的干预措施,以减少研究地区PTB的传播和抗结核耐药性的发展。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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审稿时长
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